FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
e(y)2, xmas
FlyBase ID
FBig0000117182
Interaction Network
Interactions Browser links
e(y)2 network
xmas network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0200574-10.coIP.WB
Reference
Kurshakova et al., 2007
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
e(y)2 

E(y)2

bait
xmas 

Xmas-2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kurshakova et al., 2007)

Nuclear extract was treated with DNase and RNase.

(Kurshakova et al., 2007)
FBrf0200574-11.coIP.WB
Reference
Kurshakova et al., 2007
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
xmas 

Xmas-2

bait
e(y)2 

E(y)2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kurshakova et al., 2007)

Nuclear extract was treated with DNase and RNase.

(Kurshakova et al., 2007)
FBrf0200574-9.Y2H
Reference
Kurshakova et al., 2007
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
e(y)2 

E(y)2

bait

fused to LexA DNA-binding domain

xmas 

Xmas-2

prey

fused to GAL4 activation domain

xmas 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
xmas 
interaction domain
sufficient binding region
aa 755-1370
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Kurshakova et al., 2007)

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Kurshakova et al., 2007)
FBrf0209575-11.coIP.WB
Reference
Kopytova et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
e(y)2 

ENY2

bait
xmas 

Xmas-2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Extract was treated with DNase and RNase.

(Kopytova et al., 2010)

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kopytova et al., 2010)
FBrf0209575-16.coIP.WB
Reference
Kopytova et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
xmas 

Xmas-2

bait
e(y)2 

ENY2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Extract was treated with DNase and RNase.

(Kopytova et al., 2010)

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kopytova et al., 2010)
FBrf0211509-14.coIP.WB
Reference
Gurskiy et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
xmas 

Xmas-2

bait
e(y)2 

ENY2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Gurskiy et al., 2010)
FBrf0211509-9.coIP.WB
Reference
Gurskiy et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
e(y)2 

ENY2

bait
xmas 

Xmas-2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Gurskiy et al., 2010)
FBrf0218779-10.coIP.WB
Reference
Kurshakova et al., 2009
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
xmas 

Xmas-2

bait
e(y)2 

E(y)2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kurshakova et al., 2009)
FBrf0218779-11.Y2H
Reference
Kurshakova et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
e(y)2 

E(y)2

bait

fused to LexA DNA-binding domain

xmas 

Xmas-2

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Kurshakova et al., 2009)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from cDNA expression library).

(Kurshakova et al., 2009)
FBrf0218779-9.coIP.WB
Reference
Kurshakova et al., 2009
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
e(y)2 

E(y)2

bait
xmas 

Xmas-2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kurshakova et al., 2009)
FBrf0232523-1.coIP.MS
Reference
Kopytova et al., 2016
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
e(y)2 

ENY2

bait
xmas 

Xmas-2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Crude nuclear extract was subjected to several conventional chromatographic steps and gel filtration to purify the 700 kDa TREX-2 complex. This purified fraction was then used for immunoprecipitation.

(Kopytova et al., 2016)

The interaction was not affected by treatment with DNase or RNase.

(Kopytova et al., 2016)

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kopytova et al., 2016)
FBrf0232523-14.coIP.WB
Reference
Kopytova et al., 2016
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
e(y)2 

ENY2

bait
xmas 

Xmas-2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Embryonic nuclear extract was treated with DNase and RNase.

(Kopytova et al., 2016)

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kopytova et al., 2016)
FBrf0232523-8.coIP.WB
Reference
Kopytova et al., 2016
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
xmas 

Xmas-2

bait
e(y)2 

ENY2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Embryonic nuclear extract was treated with DNase and RNase.

(Kopytova et al., 2016)

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kopytova et al., 2016)
FBrf0235940-2.coIP.WB
Reference
Popova et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
xmas 

Xmas-2

bait
e(y)2 

ENY2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Popova et al., 2016)
FBrf0235940-5.coIP.WB
Reference
Popova et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
e(y)2 

ENY2

bait
xmas 

Xmas-2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Popova et al., 2016)
External Crossreferences and Linkouts ( 0 )
References (6)