FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ecd, Pih1D1
FlyBase ID
FBig0000120930
Interaction Network
Interactions Browser links
ecd network
Pih1D1 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0248189-1.coIP.WB
Reference
Erkelenz et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
ecd 

Ecd

bait

Myc tag

ecd 
ecd 
Pih1D1 

PIH1D1

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ecd 
interaction domain
sufficient binding region
aa 1-649

coordinates relative to ecd-PA

ecd 
interaction domain residues 496,556,621
mutation decreasing interaction
aa 496,556,621

S496A,E556A,E621A, coordinates relative to ecd-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Erkelenz et al., 2021)
FBrf0248189-7.coIP.MS
Reference
Erkelenz et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
ecd 

Ecd

bait

Myc tag

Pih1D1 

PIH1D1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Erkelenz et al., 2021)
External Crossreferences and Linkouts ( 0 )
References (1)