FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Akt, yki
FlyBase ID
FBig0000121785
Interaction Network
Interactions Browser links
Akt network
yki network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0253542-1.ENZ.WB
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Akt 

Akt

enzyme

His tag

Akt 

Akt

enzyme

His tag

yki 

yki

enzyme target

GST tag

yki 
yki 

yki

enzyme target

GST tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0253542-2.ENZ.WB
Reference
Li et al., 2022
Description
physical association
Assay
Collection
Source/Stage
tracheal system / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
yki 

yki

bait

Flag tag

Akt 

Akt

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval trachea of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Li et al., 2022)

Flag tagged yki was immunoprecipitated from larval trachea; phosphorylation of yki by Akt was detected by phospho-Akt specific antibody.

(Li et al., 2022)
FBrf0253542-3.ENZ.WB
Reference
Li et al., 2022
Description
physical association
Assay
Collection
Source/Stage
tracheal system / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
yki 

yki

bait

HA tag

yki 
Akt 

Akt

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
yki 
phosphorylation target residue 168
mutation disrupting interaction
aa 168

S168A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval trachea of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Li et al., 2022)

HA tagged S168A yki was immunoprecipitated from larval trachea; phosphorylation of yki by Akt was detected by phospho-Akt specific antibody.

(Li et al., 2022)
External Crossreferences and Linkouts ( 0 )
References (1)