FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
smo, Herc4
FlyBase ID
FBig0000122304
Interaction Network
Interactions Browser links
smo network
Herc4 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0250577-1.coIP.WB
Reference
Jiang et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
smo 

Smo

bait

Myc tag

Herc4 

Herc4

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Addition of hh disrupted smo-Herc4 interaction.

(Jiang et al., 2019)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Jiang et al., 2019)
FBrf0250577-2.coIP.WB
Reference
Jiang et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Herc4 

Herc4

bait

FLAG tag

smo 

Smo

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Jiang et al., 2019)
FBrf0250577-3.coIP.WB
Reference
Jiang et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Herc4 

Herc4

bait

HA tag

smo 

Smo

prey

Myc tag

smo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
smo 
C terminal domain
sufficient binding region
aa 556-1036

coordinates relative to smo-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Jiang et al., 2019)
FBrf0250577-4.coIP.WB
Reference
Jiang et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
smo 

Smo

bait

Myc tag

smo 
smo 
Herc4 

Herc4

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
smo 
N terminal domain
sufficient binding region
aa 1-555

coordinates relative to AA-PA

smo 
SAID domain
sufficient binding region
aa 661-818

coordinates relative to AA-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Addition of Usp8 and Uch-L5 disrupted interaction of smo and Herc4.

(Jiang et al., 2019)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Jiang et al., 2019)
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References (1)