FB2025_01 , released February 20, 2025
Result: Stau_RPI_2
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General Information
Name
Stau_RPI_2
Species
D. melanogaster
Result type
RNA-protein interaction
FlyBase ID
FBlc0001676
Project
Data Provider
Title
RNA transcripts identified in stau-GFP RIP from early embryos, >2-fold enrichment.
Status
Current
Accessions
    Biosample Source
    Overview
    Strain
    Stage
    pre-blastoderm stage
    (Laver et al., 2013)
    blastoderm stage
    (Laver et al., 2013)
    gastrula stage
    (Laver et al., 2013)
    Sex
    Tissue isolated
    organism
    (Laver et al., 2013)
    Other tissues studied
    Cell component
    Cell line
    Key genes
    (transgene_used)
    Methods
    Sample preparation

    Flies were maintained at 25oC and a 0-3 hour embryo collection was collected.

    Biosamples analyzed by this result (1)
    Biosample
    Type
    Title
    BS_E1-6_stau-GFP_RIP
    whole organism
    D. melanogaster, stau-GFP line in stau mutant background, embryo (0-3 hr), source of extract for stau-GFP RIP.
    Data Analyzed
    Assay
    RIP-Seq
    (Laver et al., 2013)
    Assay methods
    Key genes
    (bait_protein)
    Protocol

    The stau-GFP protein was immunoprecipitated using a commercial anti-GFP antibody. RNA in coimmunoprecipitation was purified using Trizol reagent. Double-stranded cDNA was prepared from isolated RNA using oligo-dT primers and labelled for subsequent hybridization.

    Mode of Assay

    Labelled cDNA libraries were characterized by hybridization to custom-designed NimbleGen array (GPL10539).

    Raw Data Analyzed (1)
    Assay / Reagent collection
    Type
    Title
    RIP_E1-6_stau-GFP
    RIP-Seq
    Immunoprecipitation of RNAs (RIP) bound to GFP-tagged stau in early embryos.
    Processed Data Analyzed (0)
    Result
    Type
    Title
    Analysis
    Methods
    Reference Genome
    Reference Annotation
    Data analysis
    Comments
    Associated Data
    Size
    Files
    Additional Information
    Synonyms and Secondary IDs (2)
    Reported As
    Symbol Synonym
    Stau_RPI_2
    Name Synonyms
    RNA transcripts identified in stau-GFP RIP from early embryos, >2-fold enrichment.
    (Laver et al., 2013)
    Secondary FlyBase IDs
      References (2)