Gene or accession: CG15583
Gene annotation error
Gene CG15583 should be split.
Comments: This gene as annotated encodes at least two odorant binding
proteins. The first cDNA is
ATGCAGTCCCAATCCCTCCTGCTGATCGTTGCAGCCGTTGCCACGTTTCTGGTGGCCCAGACTACGGCCAAGTTCCTGCT
GAAGGACCACGCCGACGCAGAGAAGGCGTTCGAGGAGTGCCGTGAGGACTACTACGTGCCGGACGACATCTACGAGAAGT
ACCTGAACTACGAGTTTCCCGCCCACCGGCGCACCAGCTGCTTCGTCAAGTGCTTCCTGGAGAAGCTTGAGCTGTTCTCG
GAGAAGAAGGGATTTGACGAGCGCGCCATGATCGCCCAGTTCACCTCCAAGAGCAGCAAAGACCTGTCCACGGTCCAGCA
CGGCCTGGAGAAGTGCATCGACCACAACGAGGCCGAGTCGGATGTCTGCACCTGGGCCAACCGAGTCTTCTCCTGCTGGC
TGCCCATCAACCGCCACGTGGTGCGTAAGGTCTTCGCCTGA
encoding
MQSQSLLLIVAAVATFLVAQTTAKFLLKDHADAEKAFEECREDYYVPDDIYEKYLNYEFPAHRRTSCFVKCFLEKLELFS
EKKGFDERAMIAQFTSKSSKDLSTVQHGLEKCIDHNEAESDVCTWANRVFSCWLPINRHVVRKVFAZ
The second half is more problematic, as it appears to encode two odorant
binding proteins fused together (there is no cryptic intron to split them
apart). My best cDNA is
ATGAGTTCCCCTCGTGCGGTTCTAGTCAGCCTGTTCCTGATCTGCAGTCAGGCACTAGCTGACCTTTCTGGTGATGCCCA
GACTCTGGAAAAGTGCCTGCGGCAACTTAGTTCGCCGGAGAGCATTGCTGGCGATCTTCGAAAGCTGGAACGGTATTCAT
CTTGGACGCGGGAGGAGGTACCTTGCCTGATGCGCTGCTTGGCCAGAGAAAAGGGCTGGTTCGACGTGGAGGAAAACAAG
TGGAGGCTCAAGCAACTGACTGAGGACCTGGGCGCCGATGTCTATAACTACTGCAGATTCGAGCTGCGCCGGATGGGGTC
CGATGGCTGCAGCTTCGCCTATCGGGGACTCAGGTGCCTGAAGCAGGCCGAGATGCATGCGGGCACCAGCCTGAGCACAC
TGCTACAGTGTTCCCGCCAGCTGAACGCCACCAACGTGGAGCTGCTGCAGTACAGTAAGCTGAAGTCAAAGGAACCTATT
CCCTGCCTCTTTCAGTGCTTTGCGGATGCCATGGGATTCTACGATCCCGATGGAAACTGGCGGCTGGAAAACTGGAAGCA
GGCGTTTGGGCCTTCCGGAAATGAGGATCAGTCTTCTGGCGCTGACTACAGTGGCTGTCGACTAAGTGGGACTCAGCGGG
AGGTGGCGCTAAGCAAGTGCTCGTGGATGTACCATGAGTACAAATGCTGGGAGCGAGTAAATGGGAATAAGCTAGTGGAG
GACAACGAAGAGCAGTAG
encoding
MSSPRAVLVSLFLICSQALADLSGDAQTLEKCLRQLSSPESIAGDLRKLERYSSWTREEVPCLMRCLAREKGWFDVEENK
WRLKQLTEDLGADVYNYCRFELRRMGSDGCSFAYRGLRCLKQAEMHAGTSLSTLLQCSRQLNATNVELLQYSKLKSKEPI
PCLFQCFADAMGFYDPDGNWRLENWKQAFGPSGNEDQSSGADYSGCRLSGTQREVALSKCSWMYHEYKCWERVNGNKLVE
DNEEQZ
OBP structure has recently been resolved as a dimer, so perhaps this is a
novel evolutionary arrangement that enforces a heterodimer.