FB2025_01 , released February 20, 2025
Sequence Feature: Dmel\dsRNA-GD2750
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General Information
Symbol
Dmel\dsRNA-GD2750
Species
D. melanogaster
Feature type
RNAi_reagent
FlyBase ID
FBsf0000073330
Collection
Associated gene
Genomic Location
Chromosome (arm)
3L
Sequence location

3L:20,500,604..20,500,918 [+]

Genomic Maps
Sequence Data
Length
315
Comments

One copy of repeat shown

(Dietzl et al., 2007)

Sequence

Sequence Downloader
TCCAACCACGGCACATCACAAAATCTAGTCACAAATAAAGAGCTGGGCCAACAGTTCAACAAAGAGGAAAACCTTCAGAA
TGATTTAACTGAAAAGGAGAAAATTGATAATGGATCCCTAAACTCTTCCGATCTTTGGGAAGTGCTGCGTAAAAGTCGTA
AGGATAAGAACCTGCTCGTGATATACCTCATAACATTCCTCTCGATTTGGCCCTTTGCAGGAGTGGATTCTACTGCGCCC
GTTTATCTCAAAACTAATATGGGATTTGAGTACGAGGAGGTGTCCATGATGTTGGGATTACTATCTGTGCTGGCG
Associated Information
Gene(s) (targeted or local)
Allele(s)
    Transcripts(s)
      Polypeptide(s)
        Construct
        Experimental Data
        Progenitors
        PCR template

        genomic DNA

        (Dietzl et al., 2007)
        Primer 1 
        TCCAACCACGGCACATCACAAA
        (Dietzl et al., 2007)
        Primer 2 
        CGCCAGCACAGATAGTAATCCCAACA
        (Dietzl et al., 2007)
        Comments
        Collection Information
        Collection: VDRC-GD
        Symbol
        VDRC-GD
        Title
        A set of transgenic RNAi constructs for expression of dsRNA under UAS control, VDRC first generation.
        Source and Progenitors
        Species of derivation
        D. melanogaster
        Strain of derivation
        Vector of progenitor construct
        pP{MF3}
        Description

        Comprehensive collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene.

        (Dietzl et al., 2007)

        NOTE: Dataset members correspond to the amplicon sequence features; these can be used to retrieve associated constructs and stocks.

        (FlyBase Curators, 2013)
        Additional data
        Sample preparation
        Collection preparation

        A collection of over 13,200 RNAi transgenes (over 22,200 lines), each designed to target a single protein-coding gene; each construct contains short gene fragments cloned as inverted repeats that are expressed using the binary GAL4/UAS system. It is estimated that 88% of predicted protein-coding genes are targeted.

        (Dietzl et al., 2007)
        Mode of assay
        Data analysis

        A specificity score (Q) was calculated for each construct, such that if Q is < 0.5, off-target effects are probable and if Q > 0.8, off-target effects are improbable.

        (Dickson et al., 2007.7.18)
        Cell lines
        Observed in
        Not observed in
        Comments
        Stocks (1)
        External Crossreferences and Linkouts ( 0 )
        Synonyms and Secondary IDs (2)
        Reported As
        Symbol Synonym
        2750
        (Dietzl et al., 2007)
        dsRNA-GD2750
        Secondary FlyBase IDs
        • FBri0069905
        References (1)