FlyBase Recombinant Construct Report: P{ase-lacZF:2.0}

FB2025_01 , released February 20, 2025

FB2025_01 , released February 20, 2025

Recombinant Construct: P{ase-lacZF:2.0}

General Information

Symbol

P{ase-lacZF:2.0}

FlyBase ID

FBtp0001559

Feature type

transgenic_transposable_element

Component Allele(s)

Ecol\lacZ^ase.F20

Also Known As

ase-lacZ

Size

Expression Data

lacZ reporter

Associated insertion(s)

Molecular map

Description and Uses

Construct components

Component

Use(s)

Marker(s)

Component allele Ecol\lacZ^ase.F20

Transgenic product class

Component

Use(s)

Regulatory region(s)

Encoded product / tool

reporter enzyme

Description

1.6kb upstream of ase start site, and 455bp of ase 5' untranslated region driving expression of lacZ.

(Jarman et al., 1993)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Ecol\lacZ^ase.F20

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 10 -- 15

precursor cell of the stomatogastric nervous system

(Hartenstein et al., 1994)

delaminating stomatogastric nervous system precursor

(Hartenstein et al., 1994)

embryonic stage 10 -- 17

(Jarman et al., 1993)

embryonic stage 11 -- 17

presumptive embryonic/larval nervous system

(Jarman et al., 1993)

posterior midgut primordium

(Jarman et al., 1993)

embryonic stage 12 -- 15

tip stomatogastric nervous system precursor

(Hartenstein et al., 1994)

embryonic stage 13 -- 14

invaginating stomatogastric nervous system precursor

(Hartenstein et al., 1996)

embryonic stage 16

embryonic/larval Malpighian tubule

(Jarman et al., 1993)

embryonic/larval midgut

(Jarman et al., 1993)

third instar larval stage

(Jarman et al., 1993)

prepupal stage P1

external sensory organ precursor cell of prothoracic leg disc

(Mellert et al., 2012)

Additional Information

Statement

Reference

Expression is observed in all stomatogastric nervous system precursor cells as soon as they have lost their epithelial configuration.

(Hartenstein et al., 1994)

Expressed in all identified neural precursors.

(Jarman et al., 1993)

Marker for

invaginating stomatogastric nervous system precursor

(Hartenstein et al., 1996)

Reflects expression of

(Mellert et al., 2012, Hartenstein et al., 1996, Hartenstein et al., 1994, Jarman et al., 1993)

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

pP{ase-lacZF:2.0}

(Jarman et al., 1993)

descendant(s)

Comments

construct_comment: One of a series of lacZ reporter constructs designed to assay regulatory elements within the 5\' regulatory and promoter sequences of ase.

(Jarman et al., 1993)

Stocks (7)

Stocks containing insertion(s) of this recombinant construct

8781

wwy²/CyO, P{ase-lacZF:2.0}PK2

8757

dode¹/CyO, P{ase-lacZF:2.0}PK2

8755

y¹ w^*; robo1¹/CyO, P{ase-lacZF:2.0}PK2

5141

w^*; P{FRT(w^hs)}G13 shot³/CyO, P{ase-lacZF:2.0}PK2

8753

st¹ e¹ Hem^J4-48/TM6B, P{ase-lacZF:2.0}PK3, Tb¹

9553

w^*; ru¹ hry¹ Ptp69D⁷ Diap1^th-1 st¹ cu¹ sr¹ e^s/TM6B, P{ase-lacZF:2.0}PK3, Tb¹

108072

w^*; P{FRT(w^hs)}G13 shot³/CyO, P{ase-lacZF:2.0}PK2

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

P{ase-lacZF:2.0}

(FlyBase, 1996-)

T8LZ

(Kolodziej et al., 1995)

ase-lacZ

(Mellert et al., 2012, Pi et al., 2004, Pi et al., 2001)

Secondary FlyBase IDs

References (10)

Report Sections