Recombinant construct P{UAS-tkv.Q253D.L}

General Information
Symbol	P{UAS-tkv.Q253D.L}	FlyBase ID	FBtp0002670
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Lecuit et al., 1996)
Uses	CV term Qualifiers & info Reference binary system (targeted) tkv (Lecuit et al., 1996) characterization tkv (Lecuit et al., 1996)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Lecuit et al., 1996) chimeric gene (Lecuit et al., 1996) modified gene (Lecuit et al., 1996) selectable marker (Lecuit et al., 1996)
Component Alleles
Allele	tkv^{Q253D.Scer\UAS.cLa}
Reference(s)	(Lecuit et al., 1996, Yoshida et al., 2005, Morey et al., 2001, Dorfman et al., 2002, Wu and Cohen, 2002, Bach et al., 2003, Baena-Lopez and Garcia-Bellido, 2003, Zartman et al., 2008, Dorfman and Shilo, 2001, Franch-Marro and Casanova, 2000, Llimargas, 2000, Fernandez et al., 2000, Kim et al., 2006, Wu and Cohen, 1999, Llimargas and Casanova, 1997, Wappner et al., 1997, de Celis, 1997)
Molecular data	Scer\UAS regulatory sequences drive expression of a constitutively active form of tkv. (de Celis, 1997) Construct: Expression of a constitutively active form of tkv is driven by Scer\UAS Scer\GAL4 binding sites. (Lecuit et al., 1996)
Phenotypic class	lethal \| pupal stage \| heat sensitive, with Scer\GAL4^Ser.PGF (Kim et al., 2006) visible, with Scer\GAL4^GMR.PF (Bach et al., 2003)
Phenotype manifest in	dorsal appendage, with Scer\GAL4^CY2 (Zartman et al., 2008) dorsal branch primordium, with Scer\GAL4^btl.PS (Dorfman et al., 2002) egg operculum, with Scer\GAL4^CY2 (Zartman et al., 2008) embryonic/first instar larval cuticle, with Scer\GAL4^69B (Fernandez et al., 2000) embryonic/larval dorsal trunk, with Scer\GAL4^69B (Llimargas and Casanova, 1997) embryonic/larval dorsal trunk, with Scer\GAL4^btl.PS (Llimargas and Casanova, 1997, Wappner et al., 1997) embryonic/larval lateral trunk, with Scer\GAL4^69B (Llimargas and Casanova, 1997) embryonic/larval muscle system, with Scer\GAL4^btl.PS (Franch-Marro and Casanova, 2000) embryonic/larval visceral branch, with Scer\GAL4^btl.PS (Wappner et al., 1997) eye, with Scer\GAL4^GMR.PF (Bach et al., 2003) tracheal pit, with Scer\GAL4^btl.PS (Franch-Marro and Casanova, 2000) tracheocyte, with Scer\GAL4^69B (Llimargas and Casanova, 1997) tracheocyte, with Scer\GAL4^btl.PS (Llimargas and Casanova, 1997) wing, with Scer\GAL4⁶⁰⁴ (Morey et al., 2001) wing, with Scer\GAL4^bs-1348 (de Celis, 1997) wing, with Scer\GAL4^C-580 (de Celis, 1997) wing, with Scer\GAL4^Ser.PGF (Kim et al., 2006) wing vein \| ectopic, with Scer\GAL4⁶⁰⁴ (Morey et al., 2001) wing vein \| ectopic, with Scer\GAL4^bs-1348 (de Celis, 1997) wing vein \| ectopic, with Scer\GAL4^C-580 (de Celis, 1997)
Other information

Allele	w^+mC
Reference(s)	(Lecuit et al., 1996)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{UAST} (Lecuit et al., 1996)
descendant(s)
Comments
	construct_comment: Progenitors are those of the plasmid, pP{UAS-tkv.Q253D.L}. (Lecuit et al., 1996) report comment: tkv^Q253D produces a constitutively active form of the receptor. (Lecuit et al., 1996)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	activated UAS-tkv (Morey et al., 2001) P{UAS-tkv.Q253D.L} (FlyBase, 1996-) UAS-activated tkv (Dorfman and Shilo, 2001) UAS-tkv* (Wu and Cohen, 2002, Dorfman et al., 2002, Lecuit et al., 1996, Dorfman and Shilo, 2001, Franch-Marro and Casanova, 2000, Zartman et al., 2008) UAStkv* (Llimargas, 2000) UAS-Tkv* (Fernandez et al., 2000) UAS-tkv^Q25 (Baena-Lopez and Garcia-Bellido, 2003) UAS-tkv^QD (Bach et al., 2003, Kim et al., 2006) UAS-tkvQ253 (Llimargas, 2000)
Secondary FlyBase IDs

References ( 17 )
Research paper	Zartman et al., 2008, Dev. Biol. 322(2): 289--301 Cad74A is regulated by BR and is required for robust dorsal appendage formation in Drosophila oogenesis. [FBrf0207291] Kim et al., 2006, genesis 44(11): 541--549 Ectopic expression of Tollo/Toll-8 antagonizes Dpp signaling and induces cell sorting in the Drosophila wing. [FBrf0192910] Yoshida et al., 2005, Development 132(20): 4587--4598 DPP signaling controls development of the lamina glia required for retinal axon targeting in the visual system of Drosophila. [FBrf0187493] Bach et al., 2003, Genetics 165(3): 1149--1166 A sensitized genetic screen to identify novel regulators and components of the Drosophila janus kinase/signal transducer and activator of transcription pathway. [FBrf0167623] Baena-Lopez and Garcia-Bellido, 2003, Development 130(1): 197--208 Genetic requirements of vestigial in the regulation of Drosophila wing development. [FBrf0151950] Dorfman et al., 2002, Development 129(15): 3585--3596 Elbow and Noc define a family of zinc finger proteins controlling morphogenesis of specific tracheal branches. [FBrf0151266] Wu and Cohen, 2002, Development 129(10): 2411--2418 Repression of Teashirt marks the initiation of wing development. [FBrf0151632] Dorfman and Shilo, 2001, Development 128(6): 965--972 Biphasic activation of the BMP pathway patterns the Drosophila embryonic dorsal region. [FBrf0134583] Morey et al., 2001, Dev. Biol. 238(1): 145--156 Modulation of the Ras/MAPK signalling pathway by the redox function of selenoproteins in Drosophila melanogaster. [FBrf0139837] Fernandez et al., 2000, Genes Dev. 14(5): 604--614 The Drosophila shark tyrosine kinase is required for embryonic dorsal closure. [FBrf0126797] Franch-Marro and Casanova, 2000, Dev. Biol. 227(1): 80--90 The alternative migratory pathways of the Drosophila tracheal cells are associated with distinct subsets of mesodermal cells. [FBrf0131289] Llimargas, 2000, Development 127(20): 4407--4417 wingless and its signalling pathway have common and separable functions during tracheal development. [FBrf0129949] Wu and Cohen, 1999, Development 126(1): 109--117 Proximodistal axis formation in the Drosophila leg: subdivision into proximal and distal domains by Homothorax and Distal-less. [FBrf0106052] de Celis, 1997, Development 124(5): 1007--1018 Expression and function of decapentaplegic and thick veins during the differentiation of the veins in the Drosophila wing. [FBrf0092741] Llimargas and Casanova, 1997, Development 124(17): 3273--3281 ventral veinless, a POU domain transcription factor, regulates different transduction pathways required for tracheal branching in Drosophila. [FBrf0098299] Wappner et al., 1997, Development 124(22): 4707--4716 Interactions between the EGF receptor and DPP pathways establish distinct cell fates in the tracheal placodes. [FBrf0099737] Lecuit et al., 1996, Nature 381(6581): 387--393 Two distinct mechanisms for long-range patterning by decapentaplegic in the Drosophila wing. [FBrf0087557]