Recombinant construct P{UAS-ap::Chi.M}

General Information
Symbol	P{UAS-ap::Chi.M}	FlyBase ID	FBtp0011832
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Milan and Cohen, 1999)
Uses	CV term Qualifiers & info Reference characterization ap \| fusion construct (Milan and Cohen, 1999) Chi \| fusion construct (Milan and Cohen, 1999)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference chimeric protein (Milan and Cohen, 1999) selectable marker (Milan and Cohen, 1999)
Component Alleles
Allele	ap::Chi^{ChAp.Scer\UAS}
Reference(s)	(Milan and Cohen, 1999, Weihe et al., 2001, Yan et al., 2004, Milan and Cohen, 2000)
Molecular data	Construct: Scer\UAS regulatory sequences drive expression of a chimeric protein consisting of the N-terminal dimerisation domain of Chi (lacking the LIM interaction domain) linked to a C-terminal fragment of ap which contains the homeodomain (amino acids 270 to 469). (Milan and Cohen, 1999)
Phenotypic class	visible, with Scer\GAL4^ap-md544 (Milan and Cohen, 1999) visible, with Scer\GAL4^dpp.blk1 (Milan and Cohen, 1999)
Phenotype manifest in	wing, with Scer\GAL4^ap-md544 (Milan and Cohen, 1999) wing \| dorsal compartment, with Scer\GAL4^ap-md544 (Milan and Cohen, 1999) wing \| ventral compartment, with Scer\GAL4^dpp.blk1 (Milan and Cohen, 1999) wing margin \| ectopic, with Scer\GAL4^dpp.blk1 (Milan and Cohen, 1999)
Other information

Allele	w^+mC
Reference(s)	(Milan and Cohen, 1999)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{UAS-ap::Chi.M} (FlyBase, 1996-) UAS-ChAp (Yan et al., 2004, Weihe et al., 2001, Milan and Cohen, 2000, Milan and Cohen, 1999)
Secondary FlyBase IDs

References ( 4 )
Research paper	Yan et al., 2004, Development 131(2): 285--298 Multiple signaling pathways and a selector protein sequentially regulate Drosophila wing development. [FBrf0167529] Weihe et al., 2001, Development 128(22): 4615--4622 Regulation of Apterous activity in Drosophila wing development. [FBrf0141489] Milan and Cohen, 2000, Development 127(14): 3069--3078 Temporal regulation of Apterous activity during development of the Drosophila wing. [FBrf0128576] Milan and Cohen, 1999, Mol. Cell 4(2): 267--273 Regulation of LIM homeodomain activity in vivo: a tetramer of dLDB and apterous confers activity and capacity for regulation by dLMO. [FBrf0111430]