FlyBase Recombinant Construct Report: P{Gr66a-GAL4.D}

FB2025_01 , released February 20, 2025

FB2025_01 , released February 20, 2025

Recombinant Construct: P{Gr66a-GAL4.D}

General Information

Symbol

P{Gr66a-GAL4.D}

FlyBase ID

FBtp0014660

Feature type

transgenic_transposable_element

Component Allele(s)

Scer\GAL4^Gr66a.PD

Also Known As

Gr66a-GAL4, p[Gr66a-Gal4], p[Gr66a]-Gal4, Gr66a

Size

Expression Data

GAL4 reporter/driver

Associated insertion(s)

Molecular map

Description and Uses

Construct components

Component allele Scer\GAL4^Gr66a.PD

Transgenic product class

Component

Use(s)

Regulatory region(s)

Encoded product / tool

binary expression system - driver

Description

Gr66a promoter sequences regulate expression of a GAL4 driver.

(Dunipace et al., 2001)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Scer\GAL4^Gr66a.PD

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

gnathal ganglion | restricted

(Dunipace et al., 2001)

gustatory receptor neuron of labellum | subset

(Dunipace et al., 2001)

prothoracic leg | restricted

(Dunipace et al., 2001)

gustatory receptor neuron of prothoracic leg | subset

(Dunipace et al., 2001)

ventral cibarial sense organ neuron of proximal sensillum | subset

(Dunipace et al., 2001)

labellar taste bristle | subset

(Dunipace et al., 2001)

gustatory receptor neuron | subset

(Miyamoto and Amrein, 2008, Bray and Amrein, 2003, Dunipace et al., 2001)

dorsal cibarial sense organ neuron | subset

(Dunipace et al., 2001)

accessory pharyngeal nerve | restricted

(Dunipace et al., 2001)

labellum | restricted

(Dunipace et al., 2001)

adult labial nerve | restricted

(Dunipace et al., 2001)

gustatory receptor neuron of labellar taste bristle | subset

(Dunipace et al., 2001)

labral sense organ neuron | subset

(Dunipace et al., 2001)

high salt/bitter-sensing neuron

(Zhang et al., 2013)

chemosensory neuron of labellum

(Thorne et al., 2004)

chemosensory neuron of adult labral sense organ

(Thorne et al., 2004)

chemosensory neuron of ventral cibarial sense organ

(Thorne et al., 2004)

chemosensory neuron of mesothoracic leg

(Thorne et al., 2004)

chemosensory neuron of metathoracic leg

(Thorne et al., 2004)

chemosensory neuron of prothoracic leg

(Thorne et al., 2004)

intermediate labellar taste bristle | faint

(Hiroi et al., 2002)

intermediate labellar taste bristle I-a | faint

(Hiroi et al., 2002)

intermediate labellar taste bristle I-b | faint

(Hiroi et al., 2002)

bitter-sensitive labellar taste bristle

(Hiroi et al., 2002)

short labellar taste bristle S-c | faint

(Hiroi et al., 2002)

short labellar taste bristle | faint

(Hiroi et al., 2002)

bitter-sensitive labellar taste bristle | faint

(Hiroi et al., 2002)

prothoracic leg taste bristle chemosensory neuron | subset

(Miyamoto and Amrein, 2008)

gustatory receptor neuron | subset of labellum

(Zhang et al., 2013)

adult gnathal ganglion | restricted

(Zhang et al., 2013)

gustatory receptor neuron of the labellum

(Lee et al., 2009)

gustatory receptor neuron of the adult pharynx

(Lee et al., 2009)

abdominal 5 dorsal multidendritic neuron ddaD

(Shimono et al., 2009)

abdominal 6 dorsal multidendritic neuron ddaD

(Shimono et al., 2009)

larval abdominal lateral multidendritic neuron ldaA

(Shimono et al., 2009)

adult abdominal lateral multidendritic neuron ldaA-like

(Shimono et al., 2009)

short labellar taste bristle S-a

(Hiroi et al., 2002)

short labellar taste bristle S-b

(Hiroi et al., 2002)

adult labellum sensillum | subset

(Shim et al., 2015)

Additional Information

Statement

Reference

Drives expression in a subset of gustatory receptor neurons in the labellum, pharynx, and legs.

(FlyBase Curators, 2017)

ScerGAL4^Gr66a.PD drives expression in a subset of gustatory receptor neurons in the labellum which do not overlap with those marked by NcraQF^Ir76b.1.5. The projections of the ScerGAL4^Gr66a.PD-positive neurons in the subesophageal ganglion show minimal overlap with projection of neurons labelled by NcraQF^Ir76b.1.5.

(Zhang et al., 2013)

Gr66a and Gr93a are co-expressed in the labellum and the pharynx.

(Lee et al., 2009)

ScerGAL4^Gr66a.PD is expressed in 8 to 22 cells of each labial palp, 8 cells of the first leg, 7 cells of the second leg and 6 cells of the third leg.

(Thorne et al., 2004)

Adult expression is observed in about 20 cells, including an average of 1.6 cells in the prothoracic leg, 8 in the labial palps and in the labral and cibarial sense organs.

(Bray and Amrein, 2003)

Adult expression is observed in about 30 gustatory sensilla in the prothoracic legs, labral and cibarial sense organs, and laterally through two rows of bristles in the labial palps. Gr66a-expressing neurons project to two distinct regions in the subesophageal gangion via the labial and accessory pharyngeal nerves.

(Dunipace et al., 2001)

Marker for

high salt/bitter-sensing neuron

(Zhang et al., 2013, Mitri et al., 2009)

Reflects expression of

(Zhang et al., 2013, Zhang et al., 2013, Lee et al., 2009, Miyamoto and Amrein, 2008, Thorne et al., 2004, Bray and Amrein, 2003, Hiroi et al., 2002, Dunipace et al., 2001)

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (1)

Stocks containing insertion(s) of this recombinant construct

28801

w^*; P{Gr66a-GAL4.D}2; TI{GAL4}Gr93a³

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (15)

Reported As

Symbol Synonym

GR66a-GAL4

(Galili et al., 2014)

Gr66-GAL4

(de Haro et al., 2010)

Gr66a

(Shimono et al., 2009, Hiroi et al., 2002)

Gr66a-Gal4

(Guillemin et al., 2024, Snell et al., 2022, Li and Montell, 2021, Li et al., 2020, Zhou et al., 2019, Sánchez-Alcañiz et al., 2017, Du et al., 2016, Zhang et al., 2013, Houot et al., 2012, Kang et al., 2012, Colomb and Stocker, 2007, Ebbs and Amrein, 2007, Lacaille et al., 2007)

Gr66a:Gal4

(Miyamoto and Amrein, 2008)

Gr66a^Gal4

(Wang et al., 2011)

Gr66aGAL4

(Burke and Waddell, 2011)

P^Gr66a-GAL4

(Jiao et al., 2007)

P^Gr66a-Gal4

(López-Arias et al., 2011)

P[Gr66a-GAL4]

(Lee et al., 2015, Poudel et al., 2015)

P{Gr66a-GAL4.D}

(FlyBase, 1996-)

[66a]->Gal4

(Dunipace et al., 2001)

p[Gr66a-Gal4]

(Moon et al., 2006)

p[Gr66a]-Gal4

(Thorne et al., 2004, Bray and Amrein, 2003)

Secondary FlyBase IDs

References (56)

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