Recombinant construct P{Cyp6g1-GAL4.HR}

General Information
Symbol	P{Cyp6g1-GAL4.HR}	FlyBase ID	FBtp0023174
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	1 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Chung et al., 2007)
Uses	CV term Qualifiers & info Reference binary system (regulatory) Cyp6g1 (Chung et al., 2007)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Chung et al., 2007) selectable marker (Chung et al., 2007)
Component Alleles
Allele	Scer\GAL4^Cyp6g1.HR
Reference(s)	(Chung et al., 2007, Bogwitz et al., 2005, Norgate et al., 2006, Guittard et al., 2011, Giraudo et al., 2010, Daborn et al., 2007, McCart and Ffrench-Constant, 2008)
Molecular data	A 1608bp fragment consisting of Cyp6g1 upstream sequences to the translational start site, from the insecticide-resistant Hikone-R strain, is fused upstream of Scer\GAL4. The Cyp6g1 fragment contains the accord LTR sequence that is present in this strain. (Chung et al., 2007) Construct: Cyp6g1 promoter sequences are cloned upstream of Scer\GAL4. (Bogwitz et al., 2005)
Phenotypic class	chemical resistant, with Cyp6g1^Scer\UAS.cDa (Chung et al., 2007, Daborn et al., 2007) chemical resistant, with Cyp6g2^Scer\UAS.cDa (Daborn et al., 2007) chemical resistant, with Cyp12d1-d^Scer\UAS.cDa (Daborn et al., 2007) chemical resistant, with Cyp12d1-p^Scer\UAS.cDa (Daborn et al., 2007) lethal \| P-stage, with Cyp18a1^Scer\UAS.cGa (Guittard et al., 2011) lethal \| third instar larval stage, with Cyp18a1^Scer\UAS.cGa (Guittard et al., 2011) melanotic mass phenotype \| third instar larval stage, with Cyp18a1^Scer\UAS.cGa (Guittard et al., 2011)
Phenotype manifest in	cuticle \| third instar larval stage, with Cyp18a1^Scer\UAS.cGa (Guittard et al., 2011) midgut \| third instar larval stage, with Cyp18a1^Scer\UAS.cGa (Guittard et al., 2011)
Other information

Allele	w^+mW.hs
Reference(s)	(Chung et al., 2007)
Molecular data	A mini-w gene is expressed under the control of an Hsp70 promoter. (Heinrich et al., 1993) w gene expression is under the influence of FLIP recombinase. (Golic and Lindquist, 1989) Expression of a fragment of w genomic DNA with a 2.2kb deletion of 3' flanking sequences and a reduction in the size of the largest intron to 356bp is driven by a 388bp fragment of an Hsp70 gene. (Klemenz et al., 1987) Construct: A 'mini-white' gene constructed by deletion of the XbaI-HindII fragment from the long 5' intron. Carried by the W6 and W8 series of vectors.
Phenotypic class	suppressor of variegation, with Su(var)205⁵⁰² (Ahmad and Golic, 1996)
Phenotype manifest in	pigment cell (Kurkulos et al., 1994, Golic and Lindquist, 1989)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	5'Hikone-R,Gal4 (McCart and Ffrench-Constant, 2008) 5'HR-GAL4 (Guittard et al., 2011) 6g1HR-6c-GAL4 (Guittard et al., 2011) 6g1HR-GAL4 (Chung et al., 2007, Guittard et al., 2011, Daborn et al., 2007) 6g1HR-GAL4-6c (Bogwitz et al., 2005) Cyp6g1-GAL4 (Giraudo et al., 2010) GutSpecifc-GAL4 (Norgate et al., 2006) HR-GAL4 (Daborn et al., 2007) P{Cyp6g1-GAL4.HR} (FlyBase, 1992-)
Secondary FlyBase IDs

References ( 7 )
Research paper	Guittard et al., 2011, Dev. Biol. 349(1): 35--45 CYP18A1, a key enzyme of Drosophila steroid hormone inactivation, is essential for metamorphosis. [FBrf0212396] Giraudo et al., 2010, Pestic. Biochem. Physiol. 97(2): 115--122 Regulation of cytochrome P450 expression in Drosophila: Genomic insights. [FBrf0211123] McCart and Ffrench-Constant, 2008, Pest Manag. Sci. 64(6): 639--645 Dissecting the insecticide-resistance-associated cytochrome P450 gene Cyp6g1. [FBrf0205366] Chung et al., 2007, Genetics 175(3): 1071--1077 Cis-regulatory elements in the Accord retrotransposon result in tissue-specific expression of the Drosophila melanogaster insecticide resistance gene Cyp6g1. [FBrf0200035] Daborn et al., 2007, Insect Biochem. Mol. Biol. 37(5): 512--519 Evaluating the insecticide resistance potential of eight Drosophila melanogaster cytochrome P450 genes by transgenic over-expression. [FBrf0202087] Norgate et al., 2006, Mol. Biol. Cell 17(1): 475--484 Essential roles in development and pigmentation for the Drosophila copper transporter DmATP7. [FBrf0191161] Bogwitz et al., 2005, Proc. Natl. Acad. Sci. U.S.A. 102(36): 12807--12812 Cyp12a4 confers lufenuron resistance in a natural population of Drosophila melanogaster. [FBrf0188252]