FB2025_01 , released February 20, 2025
Recombinant Construct: P{UAS-Venus-flfl.Δ3NLS+2NES}
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General Information
Symbol
P{UAS-Venus-flfl.Δ3NLS+2NES}
FlyBase ID
FBtp0052160
Feature type
transgenic_transposable_element
Component Allele(s)
flflΔ3NLS+2NES.UAS.Venus,Tag:NES(Unk)
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele flflΔ3NLS+2NES.UAS.Venus,Tag:NES(Unk)
Transgenic product class
inframe_deletion
(Sousa-Nunes et al., 2009)
missense_variant
(Sousa-Nunes et al., 2009)
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
flfl
Tagged with
Tag:NES(Unk)
Venus
Description

UAS regulatory sequences drive expression of flfl coding sequences in which all three nuclear localization signals have been mutated. NLS1 and NLS2 have been altered using site directed mutagenesis (amino acid replacements R159A, K160A, K162A and K237A, K238A, R240A respectively) and NLS3 has been truncated. A nuclear export sequence (Tag:NES(Unk)) has been added to both the N-terminal and C-terminal ends of the protein, and it is tagged at the N-terminal end with Venus.

(Sousa-Nunes et al., 2009)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
66537
w1118; P{UAS-Venus-flfl.Δ3NLS+2NES}1
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{UAS-Venus-flfl.Δ3NLS+2NES}
(FlyBase, 1992-)
V::flflΔ3NLS+2NES
(Sousa-Nunes et al., 2009)
Secondary FlyBase IDs
    References (3)