FB2025_01 , released February 20, 2025
Recombinant Construct: P{ppk28-GAL4.C}
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General Information
Symbol
P{ppk28-GAL4.C}
FlyBase ID
FBtp0054514
Feature type
transgenic_transposable_element
Component Allele(s)
Scer\GAL4ppk28.PC
Also Known As
ppk28-Gal4
Size
Expression Data
GAL4 reporter/driver
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Scer\GAL4ppk28.PC
Transgenic product class
Component
Use(s)
Regulatory region(s)
ppk28
ppk28_promoter-Gal4
Encoded product / tool
GAL4
Description

A 1.00kb upstream fragment of ppk28 (coordinates 16699333-16700336, GenBank accession NC_004354.3) is fused upstream of sequence encoding a GAL4 driver.

(Cameron et al., 2010)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
water-sensing neuron of labellar taste bristle
(Cameron et al., 2010)
gustatory receptor neuron | subset of labellar taste bristle
(Cameron et al., 2010)
chemosensory neuron of adult labral sensillum 7 | subset
(Joseph et al., 2017)
water-sensing neuron
(Zhang et al., 2020)
Additional Information
Statement
Reference

ScerGAL4ppk28.PC drives expression in a single chemosensory neuron of adult labral sensillum 7.

(Joseph et al., 2017)

Drives expression in water-sensing neurons.

(FlyBase Curators, 2017)

ScerGAL4ppk28.PC labels the water-sensing neurons (W cell) of the taste bristles of the proboscis. Gustatory receptor neurons labeled with ScerGAL4ppk28.PC do not overlap with expression of AvicGFPGr66a.3x.IRES.2x or AvicGFPGr5a.3x.IRES.2x in the proboscis, which label the bitter and sugar-sensitive neurons, respectively. The projection of the neurons labeled with ScerGAL4ppk28.PC enter the subesophageal ganglion via the labellar nerve and the labial nerve. In the former nerve there is some overlap with projections labeled with AvicGFPGr5a.3x.IRES.2x. Almost all cells labeled with ScerGAL4ppk28.PC are also labeled by the expression of ppk28 transcript. Most taste bristles that express ScerGAL4ppk28.PC are labeled by ScerGAL4NP1017.

(Cameron et al., 2010)
 
Marker for
water-sensing neuron
(Zhang et al., 2020)
water-sensing neuron of labellar taste bristle
(Cameron et al., 2010)
Reflects expression of
ppk28
(Zhang et al., 2020, Joseph et al., 2017, Cameron et al., 2010)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
93020
w1118; Bl1/CyO; P{ppk28-GAL4.C}3
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
References (30)