FB2025_01 , released February 20, 2025
Recombinant Construct: M{UAS-Myc-smo.SD123.K851R}
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General Information
Symbol
M{UAS-Myc-smo.SD123.K851R}
FlyBase ID
FBtp0117734
Feature type
transgenic_transposable_element
Component Allele(s)
smoSD123.K851R.UAS.Tag:MYC
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Component allele smoSD123.K851R.UAS.Tag:MYC
Transgenic product class
missense_variant
(Ma et al., 2016)
polypeptide_post_translational_processing_variant
(Ma et al., 2016)
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
smo
Tagged with
Tag:MYC
Description

UAS regulatory sequences drive expression of smo in which the three PKA sites (S667, S687 and S740) and adjacent CK1 sites have been mutated to Asp (phosphomimetic mutations) and which has a mutation at the sumoylation consensus site (amino acid replacement K851R, which diminishes both basal and hh-induced sumoylation in cultured cells). The protein is tagged at the N-terminal end with six copies of Tag:MYC.

(Ma et al., 2016)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Myc-Smo<up>SD123 K851R</up>
(Ma et al., 2016)
M{UAS-Myc-smo.SD123.K851R}
(FlyBase, 1992-)
UAS-Myc-SmoSDK851R
(Ma et al., 2016)
Secondary FlyBase IDs
    References (2)