FB2025_01 , released February 20, 2025
Recombinant Construct: P{UAS-LDS-(G4C2)44.GR-GFP}
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General Information
Symbol
P{UAS-LDS-(G4C2)44.GR-GFP}
FlyBase ID
FBtp0135960
Feature type
transgenic_transposable_element
Component Allele(s)
Zzzz\GGGGCC44.LDS.UAS.GR-GFP
Also Known As
UAS-LDS-(G4C2)44
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Zzzz\GGGGCC44.LDS.UAS.GR-GFP
Transgenic product class
short_tandem_repeat_expansion
(Goodman et al., 2019)
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Zzzz\GGGGCC
Tagged with
GFP
Description

UASt regulates expression of an expanded set (44 or fewer) of repeats of GGGGCC (G4C2) - such expanded repeats of GGGGCC within the first intron of Hsap\C9orf72 are the most prominent mutation in familial Amyotrophic Lateral Sclerosis (ALS) and Frontotemporal Degeneration (FTD). This construct also contains the 114-base pair sequence immediately upstream of the repeat in intron 1 of Hsap\C9orf72 in ALS/FTD patient genomes (termed a "leader" sequence; LDS). G4C2 expansions can produce three sense-strand associated dipeptides (GA, GR, and GP), and a GFP tag (lacking an ATG initiation codon) is included 3' of the repeat in the GR-reading frame.

(Goodman et al., 2019)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
84723
w1118; P{UAS-LDS-(G4C2)44.GR-GFP}9
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (9)
Reported As
Symbol Synonym
LDS-(G4C2)44GR-GFP
(Goodman et al., 2019)
LDS-(G4C2)EXP
(Goodman et al., 2019)
P{UAS-LDS-(G4C2)44.GR-GFP}
(FlyBase, 1992-)
UAS-44R
(Cunningham et al., 2020)
UAS-LDS(G4C2)44
(Cunningham et al., 2020)
UAS-LDS-(G4C2)4,42,44[GR-GFP]
(Goodman et al., 2019)
UAS-LDS-(G4C2)44
(Gotoh et al., 2024, Sung and Lloyd, 2024)
UAS-LDS-(G4C2)44.GR-GFP
(Lee et al., 2024)
UAS-LDS-(GC42)44GR-GFP
(Fujino et al., 2023)
Secondary FlyBase IDs
    References (8)