FB2025_01 , released February 20, 2025
Recombinant Construct: P{UASp-Rho1.G14V.mClover2.nanosTCE-pgc3'UTR}
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General Information
Symbol
P{UASp-Rho1.G14V.mClover2.nanosTCE-pgc3'UTR}
FlyBase ID
FBtp0156491
Feature type
transgenic_transposable_element
Component Allele(s)
Rho1G14V.UASp.nanosTCE-pgc3'UTR.mClover2
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Component allele Rho1G14V.UASp.nanosTCE-pgc3'UTR.mClover2
Transgenic product class
missense_variant
(Lin et al., 2020)
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
Rho1
Tagged with
mClover2
Description

UASp regulatory sequences drive expression of the Rho1 coding sequence (amplified from the LD03419 cDNA clone), mutated to carry a G14V amino acid substitution (this results in a constitutively active protein). The coding sequence is tagged with mClover2. Sequence consisting of the nanos translational control element (TCE) and the pgc 3'UTR are present downstream of the coding sequence; this targets expression of the protein to the primordial germ cells.

(Lin et al., 2020)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{UASp-Rho1.G14V.mClover2.nanosTCE-pgc3'UTR}
(FlyBase, 1992-)
UASp-mClover2-Rho1-G14V-nosTCE-pgc 3'UTR
(Lin et al., 2020)
Secondary FlyBase IDs
    References (2)