Homozygotes which are maintained at the permissive temperature of 18oC have an average of 71 bristles on the basitarsus, which is slightly less than wild-type. Defective bristles are frequently found at the 8d1 and 1d1 sites (usually socketless shafts) and at bractless-bristle locations (socketless shafts or empty sockets). Homozygous prepupae shifted from 18oC to 29oC at a time equivalent to 12 hours after puparium formation at 25oC give rise to adults in which the second leg basitarsi have mostly socketless shafts at the position where bristles normally form. Some bristles are missing, and the basitarsus may be deformed and fused with the tibia. The number of complete bristles formed increases as the age at shifting to 29oC increases, reaching a plateau of approximately 68 bristles.
Males grown at 18oC are fertile. Males do not emerge when grown at 24oC or after shifts to 27oC in early or midpupal stage. Male do eclose after shifts of 96hr old pupae. Some are fertile, but become sterile after 3 days: testes are empty as no gonadal divisions have taken place and only residual motile spermatozoa are found in the seminal vesicles.
temperature-sensitive lethal. Surviving l(1)63-bearing males raised under permissive conditions exhibit precocious spermatocyte maturation in some cysts of four and eight cells; other cysts produce sixteen primary spermatocytes. Shifting such males to 27oC causes mitotic arrest leading to disappearance of gonial cells within two or three days and of primary spermatocytes after four or five days. Homozygous females raised at 18oC phenotypically normal but sterile. Mutant tergites found in gynandromorphs raised at 27oC; half-and-half gynandromorphs formed from embryos shifted from permissive to restrictive conditions 48 hr after oviposition; facet number in mutant eyes and bristles in mutant tissue reduced, indicating mitotic arrest.