FlyBase has incorporated over 10 million short guide RNA (sgRNA) designs from the DRSC. These designs represent all possible 23-mers in the D. melanogaster genome containing a 3′ proto-adjacent motif (PAM) sequence (NGG) and a 15-base 3′ sequence that is unique to the genome. These designs have been further evaluated for predicted efficiency and off-target sites at various mismatch stringencies. (More)

FlyBase has incorporated quantitative protein expression from the proteomic study of Casas-Vila et al., 2017. Measurable protein expression was obtained for over half of annotated protein coding genes (n = 8,399) in one or both of two time courses: complete life cycle (17 time points) and embryogenesis (14 time points). (More)

Around 30% of protein-coding genes in D. melanogaster encode enzymes, and at least 15% of the genome encodes factors involved in core metabolic pathways. Relevant FlyBase Gene Reports now show improved information on enzyme nomenclature and reactions, as well as links to metabolic pathway resources, which we hope will support the growing number of researchers working in these fields. (More)

Variation data from the Drosophila Genetics Reference Panel is now available as a track on JBrowse, providing information for 205 DGRP inbred lines. The track can be enabled by browsing to the “Genome Variation” section on the left panel of JBrowse and checking the box for “DGRP variants”. (More)