Has no effect on memory performance, tested 4 days after training. An olfactory classical conditioning LTM assay was used with two training protocols; a 5x training session with 15 minute rest intervals between sessions, and a 1x training session. In contrast to results reported in FBrf0080505, there was no difference between LTM assayed in these two systems between Canton S controls and CrebB-17A^a.hs, with or without heat shock.

Overexpression of CrebB-17A^a.hs in females does not affect courtship frequency. Overexpression of CrebB-17A^a.hs in females does not affect mating frequency.

Mutant flies show a slight but significant decrease in the number of rest hours per 24 hours compared to controls, even in the absence of heat shock.

Expression of CrebB^a.hs using heat shock has no significant effect on the mean daily rest rebound after rest deprivation.

Mild heat induced expression during larval stages does not alter synaptic morphology or function. Strong heat shock induction significantly increases quantal content, with little effect on bouton number. Mild induced expression in Fas2 mutants that exhibit an increase in number of boutons (Fas2^e76/Fas2^e93 transheterozygotes and Fas2^e86 homozygotes) causes a significant increase in the quantal content of the synapse without altering quantal size (as shown by measuring excitatory postsynaptic potential, EPSP), quantal content is not altered in mutants that causes an decrease (Fas2^e76 homozygotes) or no change (Fas2^e93 homozygotes) in bouton number. Expression of CrebB-17A^a.hs increases the efficacy of single boutons in Fas2^e86 to wild type.

In the absence of heat induction, no LTM was produced in wild type or CrebB-17A^a.hs flies after one, two or 10 massed training sessions. 10 spaced training sessions however produce maximal levels of LTM in both wild type and CrebB-17A^a.hs flies. Three hours after heat shock, one, two or ten massed training sessions fail to produce any LTM in wild type flies, but produced maximal levels in CrebB-17A^a.hs. CrebB-17A^a.hs has no effect on olfactory acuity, shock reactivity or learning. FlyBase curator comment: see FBrf0180428 for re-examination of these conclusions in light of discovery that CrebB-17A^a.hs actually encodes a mutant form of CrebB-17A isoform a. In contrast to results reported in FBrf0080505, FBrf0180428 reports that heat shock of CrebB-17A^a.hs was found to exert no effect on LTM in 1x training session - though assay was carried out at 5, as opposed to 7 days.