FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Alup\AFP1.9Yp2.PR
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General Information
Symbol
Alup\AFP1.9Yp2.PR
Species
A. lupus
Name
Yolk protein 2 promoter construct of Rancourt
FlyBase ID
FBal0055356
Feature type
allele
Associated gene
Alup\AFP1.9
Associated Insertion(s)
Carried in Construct
P{YP1,2;wAFP2:YP1-3'}
P{YP1,2;wAFP2}
P{YP1,2:wAFP:IVS}
Transgenic product class
wild_type
(Rancourt et al., 1990)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Rancourt et al., 1990)
Mutagen
in vitro construct
(Duncker et al., 1997, Rancourt et al., 1992, Rancourt et al., 1990)
Progenitor genotype
Carried in construct
P{YP1,2;wAFP2:YP1-3'}
P{YP1,2;wAFP2}
P{YP1,2:wAFP:IVS}
P{YP1,2:wAFP:ΔIVS}
Cytology
Description

A Yp2 promoter drives Alup\AFP1.9 expression.

(Duncker et al., 1997)

Expression of a fragment carrying Alup\AFP1.9 transcription unit and 3' untranslated sequences is driven by the Yp2 promoter from a fragment of the Yp1-Yp2 intergenic region.

(Rancourt et al., 1990)
Allele components
Component
Use(s)
Regulatory region(s)
Yp2
Encoded product / tool
Alup\AFP1.9
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Ice crystals in the haemolymph of P{YP1,2;wAFP2:YP1-3'} transgenic individuals grow into stable bipyramids. Ice crystals in nontransgenic individuals demonstrate unrestrained growth in the form of a circular plate. Adult survival trials at 0oC demonstrate that at 26 and 28 hours mortality of nontransgenic individuals is twice that seen in the transgenics, but there is no significant difference between the males and females of the transgenic line. Also males and females of the fly line Tm2 ry+ exhibit survival rates comparable to those of the transgenics at 0oC. These two lines of evidence suggest that the protective effect is not due to the transferred Alup\AFP1.5 and Alup\AFP1.9 genes.

      (Duncker et al., 1995)

      In transgenic P{YP1,2;wAFP2} individuals ice crystals are needle-shaped during thermal hysteresis (0.1-0.35oC), ice in the haemolymph of untransformed individuals grows rapidly as a flat hexagonal crystal.

      (Rancourt et al., 1990)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Female transgenic flies bearing the construct synthesize Alup\AFP1.9 and secrete it into their haemolymph, male transgenics produce no Alup\AFP1.9 and serve as controls.

      (Rancourt et al., 1990)

      Comparison of the level of mRNA produced by Alup\AFP1.5ΔIVS.Yp1 and Alup\AFP1.9Yp2.PR, a pair of transgenes transcribed in opposite orientations from the same promoter/enhancer region, suggests that the presence of intron sequences facilitate mRNA accumulation in Drosophila.

      (Duncker et al., 1997)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Alup\AFP1.9Yp2.PR
      Name Synonyms
      Yolk protein 2 promoter construct of Rancourt
      (Rancourt et al., 1990)
      Secondary FlyBase IDs
        References (4)