Two tandem copies of a 480bp eve stripe 2 enhancer fragment (containing five high-affinity bcd-binding sites) are fused to an eve promoter fragment (from -42bp to +80bp) which is separated from a kni cDNA by an FRT cassette containing an Hsp70 3' transcription termination signal. A 1.5kb eve genomic fragment containing the 3' untranslated region (UTR) is fused to the 3' end of the kni cDNA fragment.