Homozygous clones can be recovered in the eye. Females containing homozygous germ-line clones show defects in early oogenesis. The nurse cell chromosomes in the egg chambers are often strikingly underreplicated and nurse cell nuclei of older egg chambers have the morphology of younger, stage 3 wild-type chambers. When nurse cells do replicate their chromosomes they often remain polytene beyond stage 5, in contrast to wild-type chambers which lose their condensed appearance at this stage. The karyosome is sometimes incompletely condensed.