FlyBase Allele Report: Dmel\GNBP1[RNAi.UAS]

General Information

Symbol

Dmel\GNBP1^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0156444

Feature type

allele

Associated gene

Dmel\GNBP1

Associated Insertion(s)

Carried in Construct

P{UAS-GNBP1.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Pili-Floury et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Pili-Floury et al., 2004)

Mutagen

in vitro construct

(Pili-Floury et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-GNBP1.RNAi}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat of a 500bp fragment corresponding to nucleotides 122-621 of the GNBP1 coding sequence. The two copies of the 500bp fragment are separated by an intronic sequence that acts as a spacer. The RNA produced should form a hairpin.

(Pili-Floury et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

GNBP1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response, with Scer\GAL4^da.G32

(Pili-Floury et al., 2004)

Phenotype Manifest In

Detailed Description

Statement

Reference

Flies expressing GNBP1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32 show no detectable defects, indicating that GNBP1 is not essential for development. Flies expressing GNBP1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32 rapidly succumb to infection by Gram-positive bacteria (E.faecalis, S.aureus or M.luteus). Flies expressing GNBP1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32 are resistant to fungal infection (A.fumigatus or B.bassiana). Flies expressing GNBP1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32 are resistant to infection by Gram-negative bacteria (P.carotovorum.carotovorum).

(Pili-Floury et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

GNBP1^RNAi.UAS, Scer\GAL4^da.G32 has abnormal immune response phenotype, non-enhanceable by PGRP-SA^seml

(Pili-Floury et al., 2004)

NOT Enhancer of

Statement

Reference

Scer\GAL4^da.G32/GNBP1^RNAi.UAS is a non-enhancer of abnormal immune response phenotype of PGRP-SA^seml

(Pili-Floury et al., 2004)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Flies expressing GNBP1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32 in a PGRP-SA^seml background show the same degree of susceptibility to M.luteus as either PGRP-SA^seml single mutant flies or flies expressing GNBP1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32 in a wild-type background.

(Pili-Floury et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

RT-PCR confirms that overexpression of the P{UAS-GNBP1-IR} construct leads to a significant decrease in GNBP1 transcripts.

(Pili-Floury et al., 2004)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

GNBP1^RNAi.UAS

GNBP1^{dsRNA.Scer\UAS}

GNBP1^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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