FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\GluRIICUAS.cMa
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General Information
Symbol
Dmel\GluRIICUAS.cMa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Marrus
FlyBase ID
FBal0160411
Feature type
allele
Associated gene
Dmel\GluRIIC
Associated Insertion(s)
Carried in Construct
P{UAS-GluRIIC.M}
Key Links
Transgenic product class
cDNA
(Marrus et al., 2004)
wild_type
(Marrus et al., 2004)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Marrus et al., 2004)
wild_type
(Marrus et al., 2004)
Mutagen
in vitro construct
(Marrus and DiAntonio, 2004, Marrus et al., 2004)
Progenitor genotype
Carried in construct
P{UAS-GluRIIC.M}
Cytology
Description

UASt regulatory sequences drive expression of a GluRIIC cDNA.

(Marrus et al., 2004)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
GluRIIC
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Leaky expression of GluRIICUAS.cMa (without a GAL4 driver) under the control of Scer\GAL4G14 in a GluRIIC2/Df(2L)ast1 mutant background results in a significant reduction in bouton number at muscles 6/7 and 4 NMJs compared to controls.

      (Sulkowski et al., 2014)

      The ionophoretic response to glutamate at the neuromuscular junction is approximately 13-fold less than that of wild-type larvae in GluRIIC2/Df(2L)ast1 larvae carrying GluRIICScer\UAS.cMa in the absence of an Scer\GAL4 driver. In addition, these mutant animals show a 2.5-fold reduction in mean miniature excitatory junctional potential (mEJP) amplitude and a 14.4-fold reduction in mEJP frequency compared to wild type. At low calcium levels (0.47mM), there is no difference between the excitatory junctional current (EJC) amplitude of the mutant synapse and of the wild-type synapse. However, at high external calcium levels (1.5mM), the mutant synapses generate an EJC only 1/5 the size of the wild-type EJC.

      (Marrus and DiAntonio, 2004)

      Miniature excitatory junctional potentials (mEJPs) are virtually undetectable in third larval instar muscles of "weakly" rescued GluRIIC1/Df(2L)ast1 animals carrying GluRIICScer\UAS.cMa in the absence of an Scer\GAL4 driver; only rare and small events are seen.

      (Marrus et al., 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      GluRIICUAS.cMa rescues GluRIIC1/Df(2L)ast1

      (Marrus et al., 2004)
      Comments

      Leaky expression of GluRIICScer\UAS.cMa in the absence of an Scer\GAL4 driver rescues the embryonic lethality of GluRIIC1/Df(2L)ast1 animals. These "weakly" rescued animals eclose as adults and have not obvious abnormalities with locomotion, flight or fertility.

      (Marrus et al., 2004)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      GluRIICScer\UAS.cMa
      GluRIICUAS.cMa
      GluRIIIScer\UAS.cMa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Marrus
      (Marrus et al., 2004)
      Secondary FlyBase IDs
        References (5)