FlyBase Allele Report: Dmel\DCP1[b53]

General Information

Symbol

Dmel\DCP1^b53

Species

D. melanogaster

Name

FlyBase ID

FBal0219698

Feature type

allele

Associated gene

Dmel\DCP1

Associated Insertion(s)

P{lArB}DCP1^b53

Carried in Construct

Also Known As

b53

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Lin et al., 2006)

Progenitor genotype

Associated Insertion(s)

P{lArB}DCP1^b53

Cytology

Description

P{lArB} insertion 46bp downstream of the Dcp1 transcription start site, 27bp upstream of the translation start site.

(Lin et al., 2006)

Allele components

Component

Use(s)

Inserted element

P{lArB}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female semi-sterile, with DCP1^tT3

(Lin et al., 2006)

lethal | embryonic stage | germline clone | maternal effect

(Lin et al., 2006)

lethal | recessive

(Lin et al., 2006)

lethal - all die during embryonic stage | maternal effect, with DCP1^tT3

(Lin et al., 2006)

some die during embryonic stage | maternal effect, with DCP1^tT3

(Lin et al., 2006)

Phenotype Manifest In

embryo | anterior | maternal effect

(Lin et al., 2006)

embryonic abdominal segment | maternal effect

(Lin et al., 2006)

embryonic abdominal segment | maternal effect, with DCP1^tT3

(Lin et al., 2006)

primordial germ cell | maternal effect, with DCP1^tT3

(Lin et al., 2006)

Detailed Description

Statement

Reference

Homozygotes show a pleiotropic lethal phase ranging from embryonic lethality to weakly surviving adults.

Among embryos derived from females carrying homozygous germ-line clones, approximately 10% show abdominal deletions as well as deformed anterior structures. The remaining 90% of embryos die before cuticle formation.

Dcp1^b53/Dcp1^b53 ; Dcp1^tT3 individuals show a partial female sterile phenotype, with 60% of the embryos failing to hatch. Among the unhatched embryos, more than 90% show a typical posterior group phenotype, and the remaining 10% have a minor posterior group phenotype. In addition, the pole cells are reduced in number or totally missing.

(Lin et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhancer of

Statement

Reference

Dcp1[+]/DCP1^b53 is an enhancer of ventral denticle belt | germline clone phenotype of Ge-1^Δ5

(Fan et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Dcp1^b53/+ increases the penetrance of the posterior patterning phenotype of embryos derived from Ge-1^Δ5 female germline clones from 4% to 12.4%.

(Fan et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

P{Dcp1.T1} and P{Dcp1.T2} fully rescue the P{lArB}Dcp1^b53 mutant phenotype.

P{Dcp1.T3} rescues the lethality of P{lArB}Dcp1^b53 animals, however, the rescued flies show partial female sterility with a typical posterior group embryonic phenotype.

P{Dcp1.T4} cannot rescue the lethality of P{lArB}Dcp1^b53 animals, although a few barely viable but sterile adult homozygous flies can survive.

P{Dcp1.T5} rescues the lethality of P{lArB}Dcp1^b53 animals, but the rescued females show a partial female sterility.

(Lin et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

The position of the P{lArB}Dcp1^b53 insertion suggests that the mutant phenotypes may result from the disruption of Dcp1 and/or CG5602. Experiments using different rescue constructs show that a 22kDa N-terminal polypeptide of Dcp1 can rescue both the lethality and anterior phenotypes of the insertion, however a possible effect of the insertion on CG5602 cannot be ruled out. Experiments using rescue constructs confirm that the disruption of Dcp1, but not CG5602, is responsible for the posterior group phenotype of the P{lArB}Dcp1^b53 insertion.

(Lin et al., 2006)

Comments

Excision of the P{lArB}Dcp1^b53 insertion can revert the mutant phenotype.

(Lin et al., 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

DCP1^b53

Dcp1^b53

b53

(Lim et al., 2009, Lin et al., 2006)

dDcp1^b53

(Fan et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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