Most homozygotes die as larvae, with only 7% reaching the prepupal stage. Most homozygous larvae remain very small as 3rd instar larvae. A small fraction of homozygous larvae maintain normal body size but show a 1- to 2-day delay in the transition to the prepupa stage, at which point they arrest. Many of the larvae that do not prepupariate appear to have defects in moulting as evident by the appearance to two sets of mouth hooks (one corresponding to the 2nd and one to the 3rd instar).

Approximately 10% of homozygous larvae that reach the late third instar have melanotic capsules in their hemolymph.

64% of CSN8^50-2/CSN8^SH1829 animals reach prepupa stage a, but none survive beyond the prepupal stage.

CSN8^50-2/CSN8^SH1829 larvae are hypersensitive to bacterial immune challenge (after pricking them with an infected needle) compared to control larvae.

Embryos are not recovered from females carrying homozygous germ-line clones. The ovaries of these females are small and contain egg chambers that do not develop beyond stage 7.

Eyes containing homozygous clones are small, bulging from the head. The eye surface is rough and lacks the normal regular arrangement of ommatidia.