FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Ubi::EcolbirAUAS.6.Tag:BLRP
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General Information
Symbol
Dmel\Ubi::Ecol\birAUAS.6.Tag:BLRP
Species
D. melanogaster
Name
FlyBase ID
FBal0260985
Feature type
allele
Associated gene
Ubi::Ecol\birA
Associated Insertion(s)
Carried in Construct
P{UAS-Ubi6-birA}
Also Known As
UAS-(BioUb)6-BirA
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Franco et al., 2011)
Progenitor genotype
Carried in construct
P{UAS-Ubi6-birA}
Cytology
Description

UAS regulatory sequences drive expression of a synthetic linear polyubiquitin construct with Ecol\birA added at the C-terminus. Each ubiquitin chain in the construct (there are 6 copies present) has an N-terminal 16 amino acid sequence (Tag:BLRP) that can be biotinylated by Ecol\birA with high efficiency, together with a 5 amino-acid linker that provides accessibility to the tag by the Ecol\birA enzyme.

(Franco et al., 2011)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Ubi::Ecol\birA
Tagged with
Tag:BLRP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      Comments
      Comments

      The precursor protein produced from this construct (6 copies of tagged ubiquitin fused to Ecol\birA) is digested in vivo by endogenous deubiquitinating enzymes. This is followed by Ecol\birA-mediated biotinylation of the ubiquitin chains which are tagged with T:Zzzz\BLRP (a 16 amino acid residue biotinylation sequence recognised by Ecol\birA). The biotinylated ubiquitin can than be incorporated by endogenous ubiquitin-conjugating enzymes into their substrates in vivo.

      (Franco et al., 2011)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Ubi::Ecol\birAScer\UAS.6.T:Zzzz\BLRP
      Ubi::Ecol\birAUAS.6.Tag:BLRP
      Name Synonyms
      Secondary FlyBase IDs
        References (4)