FlyBase Allele Report: Dmel\Ns1[RNAi.UAS]

General Information

Symbol

Dmel\Ns1^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0282872

Feature type

allele

Associated gene

Dmel\Ns1

Associated Insertion(s)

Carried in Construct

P{UAS-Ns1.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Rosby et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Rosby et al., 2009)

Mutagen

in vitro construct

(Rosby et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-Ns1.RNAi}

Cytology

Description

UAS regulates expression of an inverted repeat of a 441bp segment of AT23067 that encodes the first 147 amino acids of Ns1, which targets Ns1 for RNAi.

(Rosby et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Ns1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate, with Scer\GAL4^da.G32

(Rosby et al., 2009)

partially lethal - majority die, with Scer\GAL4^da.G32

(Rosby et al., 2009)

Phenotype Manifest In

antenna, with Scer\GAL4^da.G32

(Rosby et al., 2009)

arista, with Scer\GAL4^da.G32

(Rosby et al., 2009)

embryonic/larval salivary gland, with Scer\GAL4^AB1

(Rosby et al., 2009)

eye, with Scer\GAL4^da.G32

(Rosby et al., 2009)

head, with Scer\GAL4^da.G32

(Rosby et al., 2009)

midgut imaginal island | larval stage, with Scer\GAL4^da.G32

(Rosby et al., 2009)

nucleolus | larval stage, with Scer\GAL4^da.G32

(Rosby et al., 2009)

Detailed Description

Statement

Reference

Scer\GAL4^da.G32 ns1^{dsRNA.Scer\UAS} individuals are slow to develop and most die during larval/pupal stages, though a few animals with the P{UAS-Ns1.RNAi}A2 insertion survive to adulthood. Many of the surviving flies display variable head, eye and antennal/aristae defects.

Expression of P{UAS-Ns1.RNAi}D1 using Scer\GAL4^da.G32 results in the loss or reduction in number of midgut imaginal island cells in larvae.

Salivary glands from Scer\GAL4^AB1 P{UAS-Ns1.RNAi}D1 larvae are significantly smaller than wild type. The number of polytene cells or imaginal ring cells is not affected, indicating that growth of polytene cells is compromised. These larvae are viable, but the resulting pupae are unable to adhere to the walls of vials.

The nucleoli of midgut epithelial (polyploid) cells in Scer\GAL4^da.G32 ns1^{dsRNA.Scer\UAS} larvae appear hypertrophied.

Malpighian tubule cells in Scer\GAL4^AB1 P{UAS-Ns1.RNAi}D1 larvae show an obvious reduction in cytoplasmic ribosomes, and extensive stacks of ribosome-free membranes.

(Rosby et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Ns1^RNAi.UAS

Ns1^{dsRNA.Scer\UAS}

Ns1^dsRNA.UAS

ns1^{dsRNA.Scer\UAS}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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