FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Avic\GFPUbi.Tag:degron(Ub-G76V)
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General Information
Symbol
Avic\GFPUbi.Tag:degron(Ub-G76V)
Species
A. victoria
Name
FlyBase ID
FBal0319944
Feature type
allele
Associated gene
Avic\GFP
Associated Insertion(s)
Carried in Construct
P{Ubi-UbG76V-GFP}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Hudson et al., 2015)
Progenitor genotype
Carried in construct
P{Ubi-UbG76V-GFP}
Cytology
Description

A ubiquitin promoter drives expression of a proteasome activity reporter protein that consists of an uncleavable ubiquitin moiety (Tag:degron(Ub-G76V)) fused to the N-terminal end of Avic\GFP. This results in the tagged protein becoming a target for poly-ubiquitylation and subsequent degradation by the ubiquitin fusion degradation pathway (details from PMID:10802622). The fusion protein can thus only accumulate when proteasome function is inhibited, and GFP fluorescence can be used as a readout of proteasome activity.

(Hudson et al., 2015)
Allele components
Component
Use(s)
Encoded product / tool
GFP
Tagged with
Tag:degron(Ub-G76V)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Avic\GFPUbi.T:Uuuu\Ub-G76V
      Avic\GFPUbi.Tag:degron(Ub-G76V)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)