Depending on the nuclear genome, near homoplasmic mt:CoII^G177S males, but not females, display a significant decrease in fertility or near sterility when reared at 25[o]C or 29[o]C of temperature, respectively, as compared to controls; mating with these males led to similar stimulation of egg production by wild-type females, but the resulting eggs are unfertilized or hatch significantly less frequently, as compared to wild-type control males.

Depending on the nuclear genome, the testes of near homoplasmic mt:CoII^G177S males, either when 3-4 days old and reared at 29[o]C or when 21 days old, but not 3-4 days old, and reared at 25[o]C, exhibit needle-stage spermatids that fail to individualize properly, remaining clumped and disorganized, and exhibit a significantly smaller seminal vesicle, which contains fewer and less motile sperm, as compared to age-matched controls; the mature sperm of 3-4 days old males raised at 29[o]C also exhibit mitochondria with significantly decreased membrane potential, as compared to controls.

Both males and females near homoplasmic for mt:CoII^G177S do not display significant differences in lifespan under 25[o]C or 29[o]C of temperature, in the time to paralysis and recovery by a 39[o]C heat shock in individuals reared at 29[o]C, in the recovery time from mechanical ('bang') shock in individuals reared at either 25[o]C or 29[o]C, or in their gender ratio, as compared to controls.