No sequence changes have been identified within the Syx1A open reading frame.
Complementation, rescue and P-element excision analysis demonstrates that the homozygous lethality of the 'SH0113' chromosome, plus the grk protein mislocalization defects seen in homozygous 'SH0113' germ-line clones, are not due to the P{lacW}SH0113 insertion (which is inserted near the wake gene), but are instead due to a second-site mutation that affects the Syx1A gene (this mutation is represented by the Syx1ASH0113 allele).