No missense or nonsense mutations have been detected in the Nacα coding region. A roo element of over 9000bp is inserted in the Nacα 3'UTR, in reverse orientation with respect to the direction of Nacα transcription.
Nacα1 was identified as a second site mutation on the sug17Δ chromosome. Deficiency mapping and rescue analysis show that the fat body cell death phenotype of the chromosome carrying Nacα1 and sug17Δ is due to the Nacα1 mutation and not the sug17Δ mutation.
