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FB2026_01
,
released March 12, 2026
CTGGAGGCCAACTAAATACTGCAAACGCCTCTACCGGGATGTGACTTCTACGAACCAAGGCAAACTCACCTGGAACACCA TCAAGCCGATTATCCAGGGACGGATCTTGTATACCCCTGCCAATGCTATGACAGAAAGCGTGGTGAAGTTTAGTAATGCC TCTTTTGAGGAACTGGATAGGCTGAAGCAACTAAGTCGTGCAGCAGCCACTATTCTCACCAAGCTGCACACAAACGCTAC TTTCCAGGAGGCCTTCGATAATCTTCTCAAGTTGGCCAAGTCGCCACTGGTCAAGAGTTTGGTCGGAGATGACTTCGATA TTGGGGAAATTGAAAGGGTCTTCGAATATATTCGCACCAACCAACTGATATATGACATTCTTACCACCGTGGCGGATTTA ATGGATTGCGTTTCGGCCGATCGTTTTGAGGCTGTGGAAAGTGTGGAGGAACTGCACAAGAGAGCCTACGAACTAAATCA AAACAAGTTGTTTTTGGCCGCCCTCAATCTGGAGGACGTAGGTCTCAAGCAAGCGTCCTACCGTCTGCATATGGATACGG ATAACACGCAGCCGACTTTTGAGAACCGGAACAGATTCTGGTTCCCCGGACCCGCCGACAGCATGGTTATTGATCTGAAG TATCATCGTGGCTTCGTTCAGCTCAAACAGATGGTCGACCTGGGAATTATCAAAAGCAAGCGAGAAGAGGCTGGCTTTGC GCCAGAGGAGGATGGGCCTGAAAGTGGGCGTTCCTTAAGTGGTCTTTTTAGTATCAAGCAGGTGGAGAACGATACGCCCG ATGAGGATGATGATGACTTTGATCTCAGTCTGGAGGAAAGCGGTGACGAGAAGGCCGCTCCAAAGGTATCAGTATCTGCG AGTGATCATGAGGCAACAACCATTTCTCCATCATCCTTGGATGGGGTAACCACTGAAGAAGTTAGGGTCACTACAGAACA GACAGTCGTAAGTGGTGACCCCGATCTTCTCCTGCTTAAAAATGAAGATGTGCTGAAGCGCTCGAAACGGCAGGGTCTGT TCGATTTGCTTGGAGGCTTCGGTGGATCTGGGGATGCCAATAAAAAGAACAAATTCGAAGTGGACAACATGCAGTTCTAC ACCAAGCAATTTCCTTATCCCGCCTTCCTTAACGATGTCTTTAAGCGTGGCTTGTACTTGGCCCAAGGTGTCCAGGTGGC TTATCTACTCGGCCTGGTGGTATTCGTCGCTCTTTCTGTAAGGGAACGCATCTGGATGCGAGAGAGCCGTAATAGCATGT TGATGCGATCAATGGGTCTAAAGGCGCATTCCGAGCTGGTAGCCTGGGCGCTGATGAGTTTTTTGGAGCTTTGTGTAATC TTTGCGCTGATCAGCGGGGTCCTGTACAGTGGCGGTATTTTGGGCTATACCAACTGGTTTTTTATGATGTTCTACTGCCT GAGCTTTGGCCTTTGCCTAATTTCGTTCTGTTATATGTGCACCAACTTTTTTAATTCGGCAAACATTGGTGCTGTGGCCT CCGCCCTTCTATTCTTCATTAGCCTTTGTCCCTTTATCATTGTGCTGATGTTCGATGCCAAATTGAGCGTTTTCGAGAGC TTCCTGGTGGACCTCTCCTTCACCACGGCCTTTGCCAAGGGCTGGGGCGAGTTGATTCGAATGGAGCTGCAGCAGGAGGG ACTGACAGTACGTCATCTCATCCAGGTGGGTCCTGCGAGAAGTGAGTGTGCCATGGCGCTGCTCATGTTCCTTCTCGATT TACTGCTGTATGCGGTTATTGGACTGGCCTACCAGCGCTATAAGAAAAATAACTACAGCTTTGTGAAGGTCAGTCGCTCA CAATTGGACGGCAAACTGGGCGCCTCGTTGGTTAATGTTAGCAAACTGTATGGCAGCAAGTGCGCTGTTTCAAACTTAAG CCTCGACCTTGCGCGCAACCAGGTGAGCTGTCTTCTGGGTCGAAATGGAGCTGGAAAGAGCACGCTGATCAAGTTGCTCA CCGGACAAATCCGGCAGAGCAGTGGCAAGGTCCTGTTGGCCGGAGAGCACCAAGTGGGTGTCTGTTGGCAGGATAATATT CTAATACCCACGCTGACGGCTCGGGAGCACCTGCAGCTGTATGCACAGATCAAGATACCACCAGGTGGGAGTGGAGGTGT GGAGGAGATCCGCTCGGAGGTTGCCCAAACCCTCCAGAGTCTAAACTTTGGTAAGCACGAATCCTATCCTTCGTGGCAGC TGTCCGGGGGCTACAGACGACGCCTCTGCGTGGCCATAGCCTTCATTGCTTCGCCCAGTGTGGTCATCTTGGATGAGCCC TGCAATGGGGTGGACGCCAAGGCGAGAAAGGACATTTGGCAGTTGATTGAGCGACTGCGTCAAGGGAGAGCAGTCATCTT TGCCACCCATTTCATGGATGAGGCCAAGTACCTCAGTGATTCACTTGTGATAATGCGGAATGGTCGCATTATTGCCCAGC ATAGTCGAGACTCCCTGCAGCGTTTGTGTACCTCAAACTATAGCATTCGATTACGTTGTGCCGATGCCACCGGAGTAACC TTCGTCATCCAGAAGGCACAGCAGCTTCTCCCTCAGACTCAGGTAACCCATTCGGGAGCCGCAGACTACCCTCACAGCCT GACCATCAATGCCAGCTATGCGGAGCATTTAACACCAGGAGCCGTCGAATTTCTGGAACTGCTGCAATCCCAAGTGACAG CTGGCAGCATCAGCGATGTGGAGCTCACAACCAGCTCAAGCCTGGAACAGGAGTTCGAGCAGTTGAATAGGAATGGCGAG GATGCGGGACCTCGACGTCCTGCCAGCGATCGGAGTGGAGTAGTCGCTGGCCCTGCAATGATAACAGACGAACCACCAAC CGCTTGCAAGCAATTCCGACTGCTCATGGGCAAGCGATTGAGGCATCTGTCCCGGAACTACCGCCTCCTTCTCTATGTCC TCCTGCTCCCAGCTCTTTTTGAACTGTGTGCCATGTGGTTCGTCAGCTATCGTCTGGAAGATGATTTCGACACTGTTCTA CCCCTGAGCCGATCGCTCTATCCCCAAACCGCTCAGTTTTTGTCACACGAGAGGGCAACCAGCTTCTCCGAGAAACTGTA TCCACAGCTAAGAACCTCATGTGACCATCTGGGCGAGTGCAGAGTATTTAATACTTCTCAACAGTCCTACGATTGGGTCC TGAACTCTTGGGGCGAGTATAGCGAGCGCCGATACGGCGGCTATGGATTAAACGGGTCAGGTGCTACAGTTTGGTATAAC AATAAGGGATATCATTCCATGATGGCCTGGCTAAACGACCTTAACTCGGAGCTACTGCGCACGACTATGAACGACTCAGA GTCTAGTATCCTTACCCTGAATGAACCGTGGAAACTGGGTTTTGCCGAACTTAGTACCAGCTCGATCCTTCGGCAGGCAG GTGACGGGTCTATGGTCTTCATCCTACTGATAGCTTTTGGCTTGGTGGTGGCATCAGGTTCCGTTTATTTGGTAAACGAG CGTGTCAATGGCGAGAAGCTGCAGCAGAGATTGTGCGGAGTAAGCGCGGTTACCTACTGGCTGGTGGCCCTCGTCTGGGA TTATCTGGTGATGGTCCTGGGTCTGATTGTCTGCCTCGTTGTAATCCTGATGTTCGGAATGCCTGTCTTTGTGGACCGCC AGCAGCTGGTGGGCATTATTGGACTGTCCCTGGCCTTTAGCTTCGCCTGTGTTCCATCTGTTCATGTGGCTGAGAAGATC TTCAGCGATTCAAGCATTGCCATTGTATCGATTTTTTGCGCCAACCTCATTGTCCCGCTGGTCACTATGGGCATCATCCT GATCCTGGGTGTGGTGGGCGATGGGCCAGCCTGGGATGATTGGCGCCACGCCCTCAACCAGGCCTTTCTGATCTTTCCAA TTCATGCTTTGGGAGACGGCTTCCTGGAGTTGTGCAAGAACTATATGGTGGCCTTGGTATTCCGCCGCTACGACATCGAC TCTTATAAGCATCCCTTGGCCAGTGACCTGCTTGGGCGCCACTTTACCGCCCTGTTGTTGGTGGGCGTGGCCGCCCTGAT CATCAACGTCCTCATCGAGTGGCATTTGCTGCGGCGTCTGTGGCAACGAGTGGAGCGATTGTTGGACTGCACCTACCGCC GCGAGCTGGATAAACTGGGTCAGCTGAAGCTGGTAAACATCCAGAGCATCTTCAAGAGCTGCGTGGACACCGGCGAAGCG GTGCGGGCGGAGAATCTCTGGCTGGCTTACCGCCGCGGACACTATGCGGTGCGTAATGTTAACTTCAGTGTCCAGCGGGG CGAATGTTTCGGGTTGCTGGGCAAGAATGGAGCAGGGAAGTCCACAATCTTCAAGCTGCTCACTGGGCAATTGCAGCCCG ACGTGGGACAAATTTACTTCGAACAACCCGGCATCTCATACTGCCCGCAGAGCAACCCGCTGGACCCGCTGCTCACGACG ACCGAGTGCATCCGCTTCTACGGACGCCTGCGCGGCATTCGTGACTTGGATCAGTTTTTGGACCGTGTGCTGGACACGTA CGAACTGCGTCCCTACAAGGATGTCCAGGTGCGAAACCTGAGTGGCGGCAATAGGCGCAAGCTAACAGTGGCCGTGACCT GTTGCGGATGTACGCCCACCGTCTTGATGGACGAACCAACGAGCGACATGGATCCCGTGACCAGGGACATGGTGTATGCC ACTATCGAACAGCTGCTGCTGGCCCGCAGAGCGGTGGTGCTGACCTCTCATTCCGTTTCGGAGATCGAGCATCTGTGCCA AAGGGTGGCGGTGCTTCGGGCGGGCCAGGTCATCGCCAGCGATAGTCCGCAGCGATTGAAATCGGAACATGGTGGTTACT ACGCCGTGACCTGCTTCTGCGGCCCCGCCCAACAGGCCATTCTTTCAAGAAGCTTGAATCAACGATTGCCGGGAGCGCGG GACTTGCAGCACTATGCCCACAGCTTGCGGTTTCTCGTCAGGGTCCGTTCGCCTGGCAGCCTGGGCGATGCCCCCCTTCT CTCCGAACTCTTCGCCATCCTTTGCGATGTCTGCGTGAATGTGGCTCGCTTTTCGTTGAGTCGCTGCCGTTTCGAGACCG TTTTCGAACGAATCCTTGACAGCAGCGAGTCGAATGGCAGCAATGGCGTGCACAAGGATCAGCAGCAACAAGTTGCTAAG AACGATCTACCAAGCAAATCCCCTGCTGTCGGTGGCACTCTTGAAACCGGCTATATTCATTGTGGCTACGAGGAGACAAG AACTTAAACGACTCAGGCATCCTCCGATTTTGAAACCAAAAACGACTATATTCAAATTCTATTTTTTAATCGAATAATGG TTATTATTCGAGACTTATTAGTGTGTTCAAAATTAAACTTTCAAAACTTGCATCAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from adult male testes and seminal vesicles.
Adult male testes and seminal vesicles were hand dissected from 0-3 day old non-isogenic Oregon-R males from a population cage.
RNA was extracted from dissected testis and poly(A)+ selected twice (kindly provided by J. Pringle and M. Fuller). cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; cDNA size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned directly into EcoRI/XhoI-digested pOTB7 plasmid. cDNAs were transformed into DH5-alpha strain (Gibco/BRL), or for AT121nn clones, into DH5-alpha TonA.