dNSF2, dNSF-2, lotus, NEM-sensitive fusion protein 2
homohexameric AAA ATPase - developmentally regulated N-ethylmaleimide-sensitive fusion (NSF) gene - mutants are defective in the synaptic growth and maturation that occurs during larval development - functions to recycle SNARE complex components subsequent to membrane fusion - required for tracheal tube growth and connectivity within the terminal cell
Gene model reviewed during 5.55
Low-frequency RNA-Seq exon junction(s) not annotated.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Nsf2 using the Feature Mapper tool.
The highest levels of Nsf2 transcripts are seen before and during cellularization. After gastrulation, expression is seen primarily in regions which give rise to ectodermal and endodermal tissues such as the midgut and the hindgut. Later, expression is also seen in the CNS. In larvae, Nsf2 expression is observed in the ring gland and in a restricted number of cells in the salivary gland. Expression is also observed in unusual patterns in the imaginal discs with the highest levels in the leg, wing and antennal discs.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Nsf2 in GBrowse 2
Maps to 3R.
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
Shows particularly robust cycling of transcription in adult heads, as assessed by expression analysis using high density oligonucleotide arrays with probe generated during three 12-point time course experiments over the course of 6 days. Shows significant change of expression pattern in circadian mutant background; decreased expression in per01, tim01 and increased expression in ClkJrk background.
comt and Nsf2 exhibit similar functional properties but have evolved distinct tissue-specific roles. comt is required in the nervous system beginning at the adult stage of development whereas Nsf2 is required in the mesoderm beginning at the first larval instar. The comt and Nsf2 proteins can functionally substitute for one another.
Evidence of multiple, highly related isoforms of NSF in a single species suggests that in some tissue membranes fusion and/or secretion has a specific role during development.