EcR-B2
fused to GAL4 DNA-binding domain
crc
fused to GAL4 activation domain
E9K
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent)
Two-hybrid system; yeast GAL4-BD/GAL4-AD
immunoprecipitated with antibody to EcR
[35]S label
R361E compensatory for EcR E9K
Interaction in vitro; bait produced by in vitro translation; prey produced and labeled by in vitro translation
Neither usp nor ecdysone was required for the EcR-crc interaction in vitro despite being required in vivo.
