FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
14-3-3ζ
FlyBase ID
FBig0000099545
Interaction Network
Interactions Browser links
14-3-3ζ network
Reported Interactions
FBrf0209680-1.CL.WB
Reference
Messaritou et al., 2010
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
14-3-3ζ 

14-3-3ζ

bait
14-3-3ζ 

14-3-3ζ

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Messaritou et al., 2010)
FBrf0209680-3.CL.WB
Reference
Messaritou et al., 2010
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
14-3-3ζ 

14-3-3ζ

bait

FLAG tag

14-3-3ζ 

14-3-3ζ

prey

FLAG tag

14-3-3ζ 
14-3-3ζ 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
14-3-3ζ 
Leu Ala Glu interaction domain
mutation decreasing interaction
aa 88-90

88AVE90 to 88NVQ90

14-3-3ζ 
Arg Val Glu and Arg Val Glu interaction domain
mutation disrupting interaction
aa 15-17,88-90

15LAE17 to 15QQR17 and 88AVE90 to 88NVQ90

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from transgenic construct; prey produced from transgenic construct.

(Messaritou et al., 2010)
FBrf0209680-4.coIP.WB
Reference
Messaritou et al., 2010
Description
physical association
Assay
Collection
Source/Stage
head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
14-3-3ζ 

14-3-3ζ

bait

FLAG tag

14-3-3ζ 

14-3-3ζ

prey
14-3-3ζ 
14-3-3ζ 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
14-3-3ζ 
Leu Ala Glu interaction domain
mutation decreasing interaction
aa 88-90

88AVE90 to 88NVQ90

14-3-3ζ 
Arg Val Glu and Arg Val Glu interaction domain
mutation disrupting interaction
aa 15-17,88-90

15LAE17 to 15QQR17 and 88AVE90 to 88NVQ90

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Messaritou et al., 2010)
FBrf0223047-1.XL.WB
Reference
Lalle et al., 2013
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
14-3-3ζ 

LEOII

self
14-3-3ζ 

LEOII

self
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult of wild-type fly line; protein produced from endogenous gene.

(Lalle et al., 2013)

14-3-3ζ homodimerizes.

(Lalle et al., 2013)

Whole fly lysates were cross-linked with BS3 reagent, and proteins were analyzed by western blot.

(Lalle et al., 2013)
External Crossreferences and Linkouts ( 0 )
References (2)