FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Reagent: FlyORF-VNshort
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General Information
Name
FlyORF-VNshort
Species
D. melanogaster
Reagent type
construct collection
FlyBase ID
FBlc0003486
Project
Created by
Vector
    Title
    A set of transgenic constructs for expression of C-terminal N-m9Venus-tagged protein coding sequences under UAS control.
    Accessions
      Overview
      Description

      A set of UAS-ORF transgenic constructs that are designed for use in a bimolecular fluorescence complementation (BiFC) assay. Each transgenic construct carries an ORF of interest tagged with a C-terminal N-m9Venus split fluorescent protein fragment and preceded by UASt regulatory sequences. The ORF is flanked by a pair of incompatible FRT sites (FRT5 and FRT2), which allows for future in vivo exchange of either the promoter or the C-terminal tag sequence. All constructs also carry the w+mC mini-white marker. Controlled expression of the ORFs can be effected by introduction of GAL4 drivers.

      (Bischof et al., 2018)
      Available from
      [FlyORF](http://flyorf.ch/index.php)
      (Bischof et al., 2018)
      Biosample Source
      Overview
      Strain
      Stage
      Sex
      Tissue isolated
      Other tissues studied
      Cell component
      Cell line
      Key genes
      Methods
      Sample preparation
      Reagent Details
      Methods
      Protocol

      Generated by an in vivo FLP recombinase-mediated swapping procedure; in each case, the C-terminal 3xTag:HA tag of an appropriate construct from the FlyORF-3 collection has been replaced with a N-m9Venus tag, together with a shortening of the linker sequence between the upstream FRT2 site and the tag.

      (Bischof et al., 2018)
      Mode of Assay
      Data analysis
      Comments

      The FlyORF-VN and FlyORF-VNshort collections differ in the length of the linker that separates the FRT2 site from the C-terminal N-m9Venus tag; the linker is 24 residues in the FlyORF-VN collection and 12 residues in the FlyORF-VNshort collection.

      (Bischof et al., 2018)
      Associated Data
      Size
      Associated features
      74 Insertion(s)
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      Additional Information
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      FlyORF-VNshort
      Name Synonyms
      A set of transgenic constructs for expression of C-terminal N-m9Venus-tagged protein coding sequences under UAS control.
      (Bischof et al., 2018)
      Secondary FlyBase IDs
        References (1)