FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Assay: RNAseq_2020_Davis_assay_Mi1_d1
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General Information
Name
RNAseq_2020_Davis_assay_Mi1_d1
Species
D. melanogaster
Assay type
RNA-Seq
FlyBase ID
FBlc0005774
Project
Data Provider
Title
Bulk RNA-sequencing of INTACT-purified nuclei from Mi1 cells
Accessions
    Biosample Source
    Overview
    Strain
    Stage
    adult stage
    (Davis et al., 2020)
    Sex
    Tissue isolated
    medulla intrinsic neuron Mi1
    (Davis et al., 2020)
    Other tissues studied
    Cell component
    Cell line
    Key genes
    Methods
    INTACT nuclei isolation
    (Davis et al., 2020)
    Sample preparation

    INTACT nuclei were typically prepared from 100 to 500 frozen fly heads. TAPIN nuclei were prepared from 100 to 3000 frozen fly heads.

    Biosamples used (1)
    Biosample
    Type
    Title
    RNAseq_2020_Davis_sample_Mi1_d1
    isolated nuclei
    INTACT-purified nuclei from Mi1 cells
    Assay Details
    Methods
    nuclear RNA isolation
    (Davis et al., 2020)
    high-throughput sequencing
    (Davis et al., 2020)
    Key genes
    Protocol

    Nuclear RNA was DNAse I treated, purified, and amplified and the cDNA was then blunted and ligated to barcoded linkers.

    Mode of Assay

    RNA-seq libraries were sequenced on an Illumina Hiseq 2500 to 50-bp read length.

    Comments
    Associated Data
    Size
    Files
    Additional Information
    Synonyms and Secondary IDs (2)
    Reported As
    Symbol Synonym
    RNAseq_2020_Davis_assay_Mi1_d1
    Name Synonyms
    Bulk RNA-sequencing of INTACT-purified nuclei from Mi1 cells
    (Davis et al., 2020)
    Secondary FlyBase IDs
      References (1)