Belikov, S.V., Belgovsky, A.I., Preobrazhenskaya, O.V., Karpov, V.L., Mirzabekov, A.D. (1993). Two non-histone proteins are associated with the promoter region and histone HI with the transcribed region of active Hsp-70 genes as revealed by UV-induced DNA-protein crosslinking in vivo.  Nucleic Acids Res. 21(4): 1031--1034.

FBrf0059177

Research paper

We described here an approach for mapping proteins on any sequence of genomic DNA. UV-induced DNA-protein crosslinking within whole cells and the 'protein image' hybridization technique (1) were applied to test the proteins bound to different regions of the D. melanogaster hsp-70 gene. The histone H1-DNA association with the coding region is shown to be maintained, even during very intensive transcription, but is absent in the promoter. Two non-histone proteins with apparent molecular masses of 50 kD (p50) and 100 kD (p100) are crosslinked only to the active hsp-70 gene regulatory region and preferentially bind to its complementary and coding DNA strands, respectively.

PMC309240 (PMC) (EuropePMC)