RE>>FlyBase needs help with some... 12/23/94
I didn't do the cytology, so I can't speak to its correctness. It is likely
that either John Belote or someone in David Ish's lab did the listed cytology.
John and I Iooked at hundreds of squashes together, so I trust his judgment
as to cytology. Your listing matches Dan Lindsley's, so I suggest staying
with it unless there is some (unknown to me) good reason to suspect its
correctness.
An additional correction not asked for in your letter. The lethal P insertion
AS416, a ry+ chorion gene insertion from Spradling's lab, maps to 92E not to
92F. I make it about 92E3-4 based on in situs with a lambda phage containing
the fragment interupted by AS416. This was originally noted by Ann Bang, then
in Jim Posakony's lab at UCSD, as part of her studies of H. We have varified
this mapping by complementation testing against Df(3R)oF4 (which complements
AS416) and Df(3R)oB16 (which uncovers AS416).
BTW: Happy Holidays to all at Flybase in the States and in Europe. You
provide and invaluable service to all of us. I'm glad to have been able to
help keep things up to date.
Mike McKeown
\--------------------------------------
To: Mike McKeown
Dear Mike
Thanks for your reply. Is the cytology we give correct ?
<up>refers to Df(3L)h-i22</up>
===========================
Subject: Re: helping flybase
RE>helping flybase 1/3/95
Rachel-
AS416 does not interrupt H. It is in fact substantially proximal to H. AS416
is a lethal and revertable insert, but it affects a different gene from H.
Ann was merely looking for potential starting points for walking to H and was
checking transposons reputed to be in 92F when she found AS416 in 92E.
Mike
\--------------------------------------
To: Mike McKeown
Dear Mike,
I am curating this portion of your message to Michael:
>An additional correction not asked for in your letter. The lethal P
insertion
>AS416, a ry+ chorion gene insertion from Spradling's lab, maps to 92E not to
>92F. I make it about 92E3-4 based on in situs with a lambda phage containing
>the fragment interupted by AS416. This was originally noted by Ann Bang,
then
>in Jim Posakony's lab at UCSD, as part of her studies of H. We have varified
>this mapping by complementation testing against Df(3R)oF4 (which complements
>AS416) and Df(3R)oB16 (which uncovers AS416).
The implication is that the insert causes a lethal allele of H. Is this so?
Happy New Year
Rachel