The eIF-2alpha gene is not on the main map (14C8-D1). Minor errors aside, FLYBASE has become a really terrific tool for our research. Thanks! Doug Cavener Subject: Re: sb and Eif Hi Doug, > Eif2alpha ... (14C8-D1) Evidently you know something we don't... how do you know that? If this is your own localisation, please send full info (was it in situ or Df mapping, etc.) and we will curate it as a pers comm. Cheers, Aubrey Subject: Re: sb and Eif Aubrey, eIF-2alpha maps to 14C8-D1 based upon the following unpublished data: 1. In situ hybridization to salivary gland chromosomes. 2. Molecular mapping relative to two P1 clones that in situ map to 14C8-D1 3. Molecular mapping relative to para; eIF-2alpha is within 20kb of the 3' end of para, which maps to 14 D1. The gene order is therefore eIF-2alpha - para - centromere. You can use the above information to update eIF-2alpha and reference as personal communication. Thanks, Doug Subject: Re: sb and Eif Thanks. The data will be curated as a pers comm; could you also please tell me the names of the two P1 clones, and which way round eIF-2alpha is (ie tandem or convergent with para)? Subject: Re: sb and Eif eIF-2alpha corresponds to STS Dm2380, Contig DS06440. This information is actually in the E of D (now defunct). (I can't run the new version of the molecular maps for some reason.) para and eIF-2alpha transcription units are in tandem. Doug Douglas R. Cavener Department of Molecular Biology Box 1820 Station B Vanderbilt University Nashville, TN 37235