Dear Rachel, I have put everything back together again, which I suppose is one up on all the Kings horses etc. XX David The latest two Cam Dps now have stock numbers and appear on our FlyBase stock list: 4744 In(2LR)DTD99LRevBR, dppd-ho cn1 bw1/CyO = Dp(2;2)Cam17 4745 In(2LR)PuLyLDTD99R/CyO = Dp(2;2)Cam18 I want to be sure all of the stocks are in good shape before I send out the announcement. I will temporarily expand the stocks, since we usually get a burst of orders whenever we post one of these announcements on bionet.drosophila. Look for the 'advertisement' in 2 to 3 weeks. Thanks for the stocks and helpful comments Kevin >The Cam Duplication Kit > >A set of large duplications covering >90% of the second chromosome is now >available from the Bloomington Drosophila Stock Center. These Dp(2;2) >stocks were generated and donated by David Gubb, Darin Coulson, Siegrun >Herrmann and John Roote of Cambridge University. These duplications are >useful in screening for dosage-dependent enhancers and suppressors of >mutant phenotypes. In addition, because the CAM duplications are nested >within inverted chromosomal segments, they act as balancer chromosomes for >the duplicated regions. This makes the CAM duplications ideal for >recovering (and maintaining) haplo-lethal, or haplo-sterile, mutations. > >Short name Stock # Duplicated region > >Dp(2;2)Cam1 3392 21E2 to 23D1 >Dp(2;2)Cam2 3394 23D1 to 26C1-2 >Dp(2;2)Cam3 3401 26C1-2 to 29E >Dp(2;2)Cam4 3403 29E to 32F >Dp(2;2)Cam5 4521 32F to 35B1-2 >Dp(2;2)Cam6 4518 35B1-2 to 36C >Dp(2;2)Cam8 2630 36C to 2Lh >Dp(2;2)Cam10 4520 2Rh to 43A1-2 >Dp(2;2)Cam11 2626 43A1-2 to 47B10-14 >Dp(2;2)Cam13 3391 47B10-14 to 48C >Dp(2;3)Cam14T 4519 49A to 51EF >Dp(2;2)Cam17 4744 52D10-E1 to 53F >Dp(2;2)Cam18 4745 53F to 57C4-6 >Dp(2;2)Cam16 2622 57C4-6 to 60E5-8> >The genotypes of these duplication stocks are given below as they appear in >the Bloomington stock list. (Note that the 'Dp(2;2)Cam' chromosome names >are used for convenience. The full designations of these aberrations >reflect their construction from recombinants between pairs of pericentric >inversions. > >3392 In(2LR)DTD8LS325R/CyO, bw1 = Dp(2;2)Cam1 >3394 In(2LR)DTD24LDTD8R, sp1/CyO, bw1 = Dp(2;2)Cam2 >3401 In(2LR)DTD111LDTD24R, bw1 sp1/CyO, bw1 = Dp(2;2)Cam3 >3403 In(2LR)DTD107LDTD111R, Dp(1;2)TE23CD, vg1/CyO, bw1 = >Dp(2;2)Cam4 >4518 In(2LR)S1Lnoc4R, cn1 bw1/CyO = Dp(2;2)Cam6 >4521 In(2LR)noc4LDTD107R, bw1 sp1/In(2LR)Gla, Gla1 Bc1 EgfrE1 >ap* cn1 bw1 = Dp(2;2)Cam5 >2630 In(2LR)C3LCH9-25R, b1/In(2L)Cy, In(2R)Cy, al2 Cy1 pr1 >Bl1 cn2 vg1 c1 sp2 = Dp(2;2)Cam8 >4520 In(2LR)TE35B-SZ4Lnoc4R, (al1) dpov1 b1 nocTE35B pr1 >cn1 bw1/CyO, Df(2L)noc20 = Dp(2;2)Cam10 >2626 (y* w*); In(2LR)TE35B-226LTE35B-4R, b1 pr1 pwn1/CyO, >b1 rk1 = Dp(2;2)Cam11 >3391 In(2LR)DTD128LTE35B-226R, Dp(1;2)TE23CD, net1 dppd-ho/CyO = >Dp(2;2)Cam13 >4519 Ts(2Lt;3Rt)6r23+Ts(2Rt;3Lt)H24, cn1/CyO = Dp(2;3)Cam14T (NB: >translocation segregants) 4744 In(2LR)DTD99LRevBR, dppd-ho cn1 bw1/CyO = Dp(2;2)Cam17 4745 In(2LR)PuLyLDTD99R/CyO = Dp(2;2)Cam18 >2622 In(2LR)ltG16LPuLyR, b1 bw1/CyO = Dp(2;2)Cam16 > >This list of stocks is also available from >http://flybase.bio.indiana.edu/stocks/stock-centers/bloomington/lk/cam-dp-kit