Subject: Helping FlyBase: ADRC-627B
Dear Brian,
We are currently curating the abstracts for the upcoming 41st
(Pittsburgh) Annual Drosophila Research Conference, for FlyBase.
I am writing in connection with your abstract:
'Variation in Immune Response in Drosophila
melanogaster at the Molecular and Phenotypic Levels.'
You mention two genes that are new to FlyBase, AttB and AttC.
Do you have a map locations for AttB and AttC? These are data items
that we track, if possible.
Another abstract, 623A (Testing the usefulness of attacin reporter
constructs as monitors of 18-wheeler activity in the humoral immune
response, by Eldon et al.) describes an attacin B. Do you know whether
this is the same gene as your AttB? If they are the same gene they
should be represented by one gene record in FlyBase, but if they are
not they should be represented by two records - which is why I ask.
Thank you for your help,
with best wishes,
Rachel.
Subject: Re: Helping FlyBase: ADRC-627B
Dear Rachel,
I would be happy to provide you with information about Attacin B and
Attacin C. I first recovered the sequence that I am referring to as
Attacin B by directly sequencing the product of a low-stringency
genomic PCR on a line deleted for Attacin A. A BLAST of the recovered
sequence against the BDGP database showed that the recovered sequence
lies 1136 bases downstream of the known Attacin A gene. BDGP also
returned a hit, which I am referring to as Attacin C, in an independent
contig. BLASTS against the Celera sequence confirmed the sequences of
the two novel genes and suggested that there are no other Attacin genes
in the D. melanogaster genome. The BDGP clone containing Attacin C
apparently maps around cytological position 50A-51A. Att A and Att B
are arranged head to tail: AttA 5'->3' -- <1.1 kb> -- AttB 5'->3'B. I
don't know the orientation of AttC relative to AttA/AttB.
.
Brian Lazzaro