Personal communication from: Kevin O'Hare, Imperial College of Science,
Technology and Medicine, London
To: Bloomington Drosophila Stock Center
Subject: P{} Insertions in sn
Dated: 24 June 1992
Background: The following information relevant to FlyBase curation was
provided with stocks contributed to the collection.
Information communicated:
snP<up>lacz,ry+1</up> \- homozygous viable and fertile, extreme bristle phenotype;
made by Jamie Paterson and Kevin O'Hare by jumping a copy of the original
O'Kane and Gehring Placz,ry+A transposon into sn
snP<up>lacz,ry+2</up> \- homozygous viable, females poorly fertile when homozygous,
extreme bristle phenotype; independent insertion made as above
snAL2 \- homozygous viable and fertile, made by Allan Lohe and Tony Mahowald,
using the Bier element
No difference in lacZ staining pattern has been detected among the three
insertions. snP<up>lacz,ry+1</up> and snP<up>lacz,ry+2</up> were mapped to the 5' exon
of sn and transcription of lacZ is in the opposite direction of that of sn. A
few blots were done with snAL2 and it does have a single insertion but that
insertion was not mapped in more detail.
The lacZ staining in adult ovaries is best defined. It begins in nuclei of
border cells at stage 8, in nurse cell nuclei at stage 9 and in the oocyte
cytoplasm at stage 11. There is some weaker staining of some follicle cells at
the posterior end of the egg chamber. The embryos begin as uniformly stained
and this becomes more diffuse as development proceeds. Larval stages show no
specific staining. There is considerable staining during pupal stages and
there is some staining in adult testes.