Subject: Helping FlyBase: ADRC-10072
Dear Rebeccah,
We are currently curating the abstracts for the upcoming 45th
(Washington DC) Annual Drosophila Research Conference, for FlyBase.
I am writing in connection with your abstract:
Characterization of Esc-like, an ESC paralog
You mention a gene symbol that is new to FlyBase, escl. Do you know
which of the Genome Project CG annotations your gene corresponds to?
All the CGs have corresponding gene records in FlyBase already and we
don't like to make duplicate records for what is actually the same gene
unless we can't avoid it. The CG symbol becomes a synonym when an annotation is
named with a more descriptive or functional name.
With best wishes,
Rachel.
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Rachel Drysdale, Ph.D.
FlyBase (Cambridge),
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Subject: Re: FW: Helping FlyBase: ADRC-10072
Hi Rachel,
Rebeccah passed your request on to me. We found 'esc-like' (CG5202)
the week the genome sequence was released based on its high degree of
protein sequence similarity to ESC. We raised antibodies to it and
subsequently named it esc-like (escl) based not only on it high
amino acid sequence similarity to ESC, but also based on our
discovery that the ESCL protein is present in a complex in vivo with
the same proteins that are also found in the ESC-E(Z) complex (namely
E(Z), SU(Z)12, p55/Caf1-p55, RPD and PCL). The ESCL complex appears
to be an 'alternative' ESC complex and we have functional evidence
that it is involved in PcG silencing. It also co-localizes with these
E(Z), SU(Z)12 and PCL at many sites on polytene chromosomes. This
work will be reported soon. Related papers from our lab on ESC are
below.
Peter Harte
Tie, F., Furuyama, T. and Harte, P.J. (2001) The Drosophila Polycomb
Group proteins ESC and E(Z) are present in a complex containing the
histone-binding protein p55 and the histone deacetylase RPD3.
Development  128:275-86 .
Furuyama, T., Tie, F. and Harte P.J. (2003) Polycomb group
proteins ESC and E(Z) are present in multiple distinct complexes that
undergo dynamic changes during development. Genesis  5:114-24 .
Tie, F., Prasad-Sinha, J., Birve, A., Rasmuson-Lestander, Å. and
Harte, P.J. (2003) A 1 MDa ESC/E(Z) complex from Drosophila that
contains the Polycomblike, and RPD3 proteins. Mol. Cell. Biol. 23:
3352-62
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Peter Harte
Dept. of Genetics
School of Medicine
Case Western Reserve University
10900 Euclid Ave.(BUT FOR COURIER SERVICES USE: 2109 Adelbert Rd.)
Cleveland, OH 44106-4955