Isolation and characterization of Df(3R)BSC137
Rachel Andrade, Jill Gresens, and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC137 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f04700 and P{XP}d00714. The deletion was isolated as a  chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6B females crossed to P{hsFLP}1, y1 w1118; PBac{WH}f04700/P{XP}d00714 males.  The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC137 from the segment of PBac{WH}f04700 to the left of its FRT site and the segment of P{XP}d00714 to the right of its FRT site. Exelixis, Inc. determined the insertion site of P{XP}d00714 to be at Release 3 genome coordinate 19422051 on chromosome arm 3R. Polytene chromosome squashes confirmed the presence of the deletion and showed the cytological breakpoints of Df(3R)BSC137 to be 95A2-4;95A8-B1.