Isolation and characterization of Df(3L)BSC181
Jill Gresens and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC181 was isolated as a FLP recombinase-induced recombination event involving P{XP}d02102 and PBac{WH}CG1919f01099. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; P{XP}d02102/PBac{WH}CG1919f01099 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC181 from the segment of P{XP}d02102 to the left of its FRT site and the segment of PBac{WH}CG1919f01099 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2R)BSC181 predicted from the Release 4 genomic coordinates of the transposable element insertion sites are 62A11;62B7. Df(3L)BSC181 failed to complement Cdc37e4D.