Subject: Helping FlyBase: ADRC-60448 and 60450 Dear Takefumi and Yoshiko, We are currently curating the abstracts for the upcoming 48th (Philadelphia) Annual Drosophila Research Conference, for FlyBase. I am writing in connection with your abstracts: polycistronically-encoded small peptides regulate actin-based morphogenesis in Drosophila. and The role of a short peptide gene, polished rice, during imaginal development. You mention a gene symbol that is new to FlyBase, pri. Do you know which of the CG annotations your gene corresponds to? All the CGs have corresponding gene records in FlyBase already and we don't like to make duplicate records for what is actually the same gene unless we can't avoid it. The CG symbols become synonyms when an annotation is named with a more descriptive or functional name. Best regards, Rachel. Subject: Re: Helping FlyBase: ADRC-60448 and 60450 Dear Rachel, Thank you for e-mail. polished rice is annotated as a non-coding RNA gene and corresponding to CR33327 in Flybase, which is based on a EST clone LD11162. Our results clearly showed that CR33327 indeed polycistronic peptide gene (it is the first case in Drosophila) and encode at least four tiny peptides. These peptides are encoded in: 408 to 443 (length = 36) 521 to 556 (length = 36) 628 to 663 (length = 36) 753 to 851 (length = 99) Each number is corresponding to that in the LD11162 sequence. This gene was also briefly described as non-coding RNA by Inagaki et al. <up>Genes. Cells, 2005, 10 (12), 1163-1173</up> and Tupy et al. <up>Proc Natl Acad Sci U S A, 2005, 102(15), 5495-5500</up>, referred to as MRE29 and npcr001:3R , respectively. When you describe polished rice, please register these synonyms to avoid confusion. We really appreciate your great efforts to keep Flybase in shape. -- Yuji Kageyama, PhD PREST Investigator Japan Science and Technology Agent Assistant Professor Graduate School of Biological Sciences Nara Institute of Science and Technology 8916-5 Takayama Ikoma, Nara 630-0192, Japan