Isolation and characterization of Df(2R)BSC335
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC335 was isolated as a FLP recombinase-induced recombination event involving P{XP}d09298 and PBac{WH}Cyp12b2f06569. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}d09298/PBac{WH}Cyp12b2f06569 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC335 from the segment of P{XP}d09298 to the left of its FRT site and the segment of PBac{WH}Cyp12b2f06569 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2R)BSC335 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 55C6;55F1. It failed to complement Df(2R)ED3683 and Df(2R)ED3636. Df(2R)FDD-0104658 is a synonym for Df(2R)BSC335.